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Differential responses of popcorn under Bipolaris maydis stress using Label Free Proteomics approach

ABSTRACT: In maize crops Southern Leaf Blight (SLB) is a severe disease caused by the fungus Bipolaris maydis (Y. Nisik & C. Miyake) leading to several losses in crop production. Unlike in maize, popcorn resistant varieties are not yet commercially available and this trait have been the subject of many popcorn breeding programs. The present study aimed to identify differentially accumulated proteins (DAPs) associated with resistance to B. maydis in two contrasting popcorn inbred lines using the comparative proteomic analysis. Forty-day old popcorn plants from resistant and susceptible inbred lines at the V4 growth stage were inoculated with B. maydis by spraying the conidium suspension. At four and ten days after inoculation, the morphological aspects of lesions in leaves were checked and the proteomic analysis was performed. Our results showed that the resistant inbred line exhibited minor foliar lesions in comparison with the SLB-susceptible genotype. In the genotype’s comparison, 644 DAPs were identified at 4 days and 613 at 10 days after B. maydis inoculation. Besides that, in resistant plants were identified DAPs upaccumulated related to response to stress, response to stimulus, photosynthesis, cellular growth, maintenance and detoxification process, which might be involved in plant response against the pathogen. It was identified proteins as salicylic acid-related and nucleotide-binding leucine-rich repeat that may be responsible to initiate a response to fungus in SBL-resistant inbred line. A differential response was identified in chloroplast proteins that is the main organelle involved in perception of the fungus to initiate a signalizing response. The changes in the proteomic profile in resistant inbred line may be effective in the response under B. maydis infection. This is the first work presenting the proteomic profile alteration under B. maydis inoculation and these findings may be useful for identifying candidate biomarkers in popcorn resistance to B. maydis being relevant for further researches on genetic breeding to develop resistant genotypes.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Leaf, Diploid Cell

SUBMITTER: Vanildo Silveira

LAB HEAD: Vanildo Silveira

PROVIDER: PXD016768 | Pride | 2025-02-03

REPOSITORIES: pride

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			Action	DRS
	20190502-092442_quantification_report.xlsx	Xlsx
	Project.xml	Xml
	Sample_10_15505122571270_7426836813328256.zip	Other
	Sample_10_popcorn_susceptible_genotype_at_10_days_after_B_maydis_inoculation.raw.zip	Raw
	Sample_11_15505122571610_19763957931793175.zip	Other

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Project description:Fusarium verticillioides is a detrimental fungus that can contaminate maize grains with mycotoxins that are harmful to human and animal health. Breeding and growing resistant genotypes is one alternative to reduce contamination and subsequent production of mycotoxins by this fungus. However, little is known about the resistant mechanism relevant to breeding in this pathosystem. Therefore, our aim was to identify genes and metabolites that may be related to Fusarium ear rot resistance using resistant and susceptible maize inbreds. Kernels of the resistant inbred showed significantly reduced disease severity, and reduced levels of total fumonisin and ergosterol content compared with the susceptible one. Gene expression data were obtained from microarray hybridizations using F. verticillioides inoculated and non inoculated maize kernels. Differentially expressed sequences were identified and classified into 36 functional categories. Most of the differentially expressed genes were assigned to the categories “protein, RNA, DNA, stress, transport, signaling and cell metabolism”. These genes encode for PR proteins, detoxification and primary metabolism enzymes. Fungal inoculation did not produce considerable changes in gene expression and metabolites in the resistant L4637 inbred, probably due to a preformed or constitutive resistance mechanism. Defense-related genes were induced or repressed in kernels of the susceptible inbred L4674, responding specifically to the pathogen infection. The qRT-PCR in infected silks showed that glucanase, lipid transfer, xylanase inhibitor, PR1 and 26S proteosome transcripts had higher expression ratios in the susceptible line compared to the resistant one in response to fungal infection. Through this study, a global view of differential genes expressed and metabolites concentration during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize. Global view of differential genes expressed during resistance and susceptibility to F. verticillioides inoculation. Two maize inbred lines : one resistant (L4637) and one susceptible (L4674) to F. verticillioides infection. Two-condition experiment, Inoculated (I) vs. non-inoculated (NI) lines. Biological replicates: 3 . One replicate per array.

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Differential responses of popcorn under Bipolaris maydis stress using Label Free Proteomics approach

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