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Digestion of a variety of synthetic peptides by proteasomes

ABSTRACT: Proteasomes are large multi-subunit enzymes that act as the main producers of antigenic peptides presented at the cell surface to CD8+ T cells. They can simply cut proteins or recombine their fragments thereby generating novel sequences, i.e. spliced peptides via a process called proteasome-catalysed peptide splicing (PCPS). In order to gain more insight into these processes, we here perform in vitro digestions of 55 synthetic polypeptide substrates by proteasome isoforms followed by LC-MS/MS measurements using different Mass Spectrometers.

INSTRUMENT(S): LTQ Orbitrap XL, Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Artem Mansurkhodzhaev

LAB HEAD: Juliane Liepe

PROVIDER: PXD016782 | Pride | 2020-04-14

REPOSITORIES: Pride

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			Action	DRS
	FileDescriptionPride.csv	Csv
	TSN18_CN23_OrbitrapXL_112817_1.raw	Raw
	TSN18_CN23_OrbitrapXL_112817_1_FT_searchResults.csv	Csv
	TSN18_CN23_OrbitrapXL_112817_1_IT_searchResults.csv	Csv
	TSN18_CN23_OrbitrapXL_112817_2.raw	Raw

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Publications

Large database for the analysis and prediction of spliced and non-spliced peptide generation by proteasomes.

Specht Gerd G Roetschke Hanna P HP Mansurkhodzhaev Artem A Henklein Petra P Textoris-Taube Kathrin K Urlaub Henning H Mishto Michele M Liepe Juliane J

Scientific data 20200515 1

Proteasomes are the main producers of antigenic peptides presented to CD8<sup>+</sup> T cells. They can cut proteins and release their fragments or recombine non-contiguous fragments thereby generating novel sequences, i.e. spliced peptides. Understanding which are the driving forces and the sequence preferences of both reactions can streamline target discovery in immunotherapies against cancer, infection and autoimmunity. Here, we present a large database of spliced and non-spliced peptides gen ...[more]

PMID: 32415162

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Project description:Proteasomes are large multi-subunit enzymes that act as the main producers of antigenic peptides presented at the cell surface to CD8+ T cells. They can simply cut proteins or ligate non-contiguous peptide fragments thereby generating novel sequences, i.e. spliced peptides via a process called proteasome-catalysed peptide splicing (PCPS). In order to benchmark methods for spliced peptide identification, we here perform in vitro digestions of TSN2 and TSN89 synthetic polypeptide substrates by K562-derived 20S proteasome followed by LC-MS/MS measurements using Orbitrap Fusion Lumos mass spectrometer. Michele Mishto, Head of the research group Molecular Immunology at King’s College London and the Francis Crick Institute, London (UK). Email: michele.mishto@kcl.ac.uk

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