Proteomics

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Mass spectrometry-based characterization of Toxoplasma gondii MORC interactome

ABSTRACT: T. gondii has a complex life cycle typified by an asexual development taking place in vertebrate, and a sexual reproduction which occurs exclusively in felids and thereby is less studied. The developmental transitions rely on changes in gene expression patterns, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified T. gondii microrchidia (MORC) protein as an upstream transcriptional repressor of sexual commitment. We performed affinity purification coupled to tandem mass spectrometry analyses to identify its binding partners.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Toxoplasma Gondii Pru Toxoplasma Gondii Rh

SUBMITTER: Yohann Couté  

LAB HEAD: Virginie Brun

PROVIDER: PXD016846 | Pride | 2020-01-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
MORC-interactome_Pru.mzid.gz Mzid
MORC-interactome_Pru.pride.mztab.gz Mztab
MORC-interactome_RH.mzid.gz Mzid
MORC-interactome_RH.pride.mztab.gz Mztab
QEx2_001105.mgf Mgf
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Publications

Toxoplasma gondii has a complex life cycle that is typified by asexual development that takes place in vertebrates, and sexual reproduction, which occurs exclusively in felids and is therefore less studied. The developmental transitions rely on changes in the patterns of gene expression, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified the T. gondii microrchidia (M  ...[more]

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