Proteomics

Dataset Information

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Peptide mapping of SRSF7_RS-GFP isoforms in murine P19 cells


ABSTRACT: Here we mapped peptides of mouse SRSF7_RS-GFP purified from P19 cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Michaela Müller-McNicoll

PROVIDER: PXD016871 | Pride | 2020-05-27

REPOSITORIES: Pride

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Publications


SRSF7 is an essential RNA-binding protein whose misexpression promotes cancer. Here, we describe how SRSF7 maintains its protein homeostasis in murine P19 cells using an intricate negative feedback mechanism. SRSF7 binding to its premessenger RNA promotes inclusion of a poison cassette exon and transcript degradation via nonsense-mediated decay (NMD). However, elevated SRSF7 levels inhibit NMD and promote translation of two protein halves, termed Split-ORFs, from the bicistronic SRSF7-PCE transc  ...[more]

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