Proteomics

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Proteomic analysis of the proteins secreted by the infection cushions of Botrytis cinerea.

ABSTRACT: Gray mold disease affects fruits, vegetables and ornemental plants around the world, causing considerable losses every year. Its causing agent, the fungus Botrytis cinerea, produces infection cushions (IC) that are complex appressorial structure dedicated to the penetration of the plant tissues. A microarray analysis identified 1,231 up-regulated genes in IC-enriched mycelium, among which several genes coding for putative secreted proteins. These data were supported by qPCR analysis in vitro and in planta. They highlight a secretion of proteins involved in virulence like proteases, plant cell wall degrading enzymes and necrosis inducers. To validate these transcriptomic data, a proteomic analysis of the IC exo-proteome is required.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Botryotinia Fuckeliana (strain B05.10) (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Nathalie Poussereau

PROVIDER: PXD016885 | Pride | 2021-06-10

REPOSITORIES: Pride

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Dataset's files

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Action DRS
Botrytis_cinerea.ASM83294v1.fasta Fasta
Linkbetweenfiles.xlsx Xlsx
cr160304_01.msf Msf
cr160304_01.pdResult Other
cr160304_01.raw Raw
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Publications

The necrotrophic plant-pathogen fungus Botrytis cinerea produces multicellular appressoria dedicated to plant penetration, named infection cushions (IC). A microarray analysis was performed to identify genes upregulated in mature IC. The expression data were validated by RT-qPCR analysis performed in vitro and in planta, proteomic analysis of the IC secretome and biochemical assays. 1231 upregulated genes and 79 up-accumulated proteins were identified. The data support the secretion of effectors  ...[more]

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