Genome-scale transcription-translation mapping reveals features of Zymomonas mobilis transcription units and promoters
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ABSTRACT: Limited functional annotation of the Z. mobilis genome is a current barrier to both basic studies of Z. mobilis and its development as a synthetic-biology chassis. To gain insight, we collected sample-matched multiomics data including RNA-seq, transcription start site sequencing (TSS-seq), termination sequencing (term-seq), ribosome profiling, and label-free shotgun proteomic mass spectrometry across different growth conditions to improve annotation and assign functional sites in the Z. mobilis genome. Proteomics and ribosome profiling informed revisions of protein-coding genes, which included 44 start codon changes and 42 added proteins.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Bacteria Zymomonas Mobilis Subsp. Mobilis Zm4 = Atcc 31821
SUBMITTER: Jessica Vera
LAB HEAD: Robert Landick
PROVIDER: PXD016962 | Pride | 2020-07-10
REPOSITORIES: Pride
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