Proteomics

Dataset Information

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Genome-scale transcription-translation mapping reveals features of Zymomonas mobilis transcription units and promoters


ABSTRACT: Limited functional annotation of the Z. mobilis genome is a current barrier to both basic studies of Z. mobilis and its development as a synthetic-biology chassis. To gain insight, we collected sample-matched multiomics data including RNA-seq, transcription start site sequencing (TSS-seq), termination sequencing (term-seq), ribosome profiling, and label-free shotgun proteomic mass spectrometry across different growth conditions to improve annotation and assign functional sites in the Z. mobilis genome. Proteomics and ribosome profiling informed revisions of protein-coding genes, which included 44 start codon changes and 42 added proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Bacteria Zymomonas Mobilis Subsp. Mobilis Zm4 = Atcc 31821

SUBMITTER: Jessica Vera  

LAB HEAD: Robert Landick

PROVIDER: PXD016962 | Pride | 2020-07-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ProteomicsSampleKey.txt Txt
ZM4.CDS.fa Other
andromeda.zip Other
ash_20170414_YZ_SAMPLE_1.raw Raw
ash_20170414_YZ_SAMPLE_10.raw Raw
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