Proteomics

Dataset Information

0

The role of Bud23 in mouse heart tissue

ABSTRACT: Efficient mitochondrial function is required in tissues with high energy demand such as the heart, and mitochondrial dysfunction is associated with cardiovascular disease. Expression of mitochondrial proteins is tightly regulated in response to internal and external stimuli. Here we identify a novel mechanism regulating mitochondrial content and function, through BUD23-dependent ribosome generation. BUD23 was required for ribosome maturation, normal 18S/28S stoichiometry and modulated the translation of mitochondrial transcripts in human A549 cells. Deletion of Bud23 in murine cardiomyocytes reduced mitochondrial content and function, leading to severe cardiomyopathy and death. We discovered that BUD23 selectively promotes ribosomal interaction with low GC-content 5’UTRs. Taken together we identify a critical role for BUD23 in bioenergetics gene expression, by promoting efficient translation of mRNA transcripts with low 5’UTR GC content. BUD23 emerges as essential to mouse development, and to postnatal cardiac function.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Maria Voronkov  

LAB HEAD: David William Ray

PROVIDER: PXD017019 | Pride | 2020-01-16

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20180405_RayD_PoolmanT_MV1.raw Raw
20180405_RayD_PoolmanT_MV2.raw Raw
20180405_RayD_PoolmanT_MV3.raw Raw
20180405_RayD_PoolmanT_MV4.raw Raw
20180405_RayD_PoolmanT_MV5.raw Raw
Items per page:
1 - 5 of 9
altmetric image

Publications

Efficient mitochondrial function is required in tissues with high energy demand such as the heart, and mitochondrial dysfunction is associated with cardiovascular disease. Expression of mitochondrial proteins is tightly regulated in response to internal and external stimuli. Here we identify a novel mechanism regulating mitochondrial content and function, through BUD23-dependent ribosome generation. BUD23 was required for ribosome maturation, normal 18S/28S stoichiometry and modulated the transl  ...[more]

Similar Datasets

Transcriptomics Cardiac mitochondrial function depends on BUD23 mediated ribosome programming
2020-01-23 | E-MTAB-8673 | biostudies-arrayexpress
Transcriptomics A systematic view on Influenza induced host shut-off
2016-08-15 | GSE82232 | GEO
Genomics Analysis of the Chlamydomonas mitochondrial ribosome RNA content
| PRJNA718484 | ENA
Proteomics Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
| MSV000092843 | MassIVE
Transcriptomics Csk restrains BCR-mediated ROS production and contributes to germinal center selection and affinity maturation
2024-05-06 | GSE244802 | GEO
Genomics Cardiac mitochondrial function depends on BUD23 mediated ribosome programming
| PRJEB36241 | ENA
Transcriptomics Transcriptome-wide mapping of human Staufen1 binding sites
2013-11-25 | E-GEOD-52447 | biostudies-arrayexpress
Other Translation-dependent and independent mRNA decay occur through mutually exclusive pathways that are defined by ribosome density during T Cell activation [Ribo-Seq]
2024-03-01 | GSE159300 | GEO
Other Translation-dependent and independent mRNA decay occur through mutually exclusive pathways that are defined by ribosome density during T Cell activation [PAS-Seq]
2024-03-01 | GSE159299 | GEO
Transcriptomics Translation-dependent and independent mRNA decay occur through mutually exclusive pathways that are defined by ribosome density during T Cell activation [RNA-Seq]
2024-03-01 | GSE159295 | GEO