Proteomics

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Antibacterial properties and mechanism of action for serum obtained from the Chinese alligator (Alligator sinensis)


ABSTRACT: Chinese alligator (Alligator sinensis) is one of the rarest endangered reptiles found in China and possesses strong immune potential. This study tested the antibacterial ability of Chinese alligator serum (CAS) against Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and analyzed the underlying mechanisms. Results showed that the CAS had a marked antibacterial effect on K. pneumoniae, E. coli, and P. aeruginosa. However, S. aureus was only mildly affected, and this effect disappeared when incubated with Protease K. The serum proteome revealed that the antibacterial ability of CAS was produced by interactions between various proteins and that the complement proteins played a major antibacterial role. Furthermore, the prediction of the structure and function of complement component 3 revealed eight potential protein binding sites and one nucleic acid binding site that were likely related to the broad-spectrum antibacterial ability of this serum. This study provided evidence that CAS elicits significant antibacterial effects against some pathogens and provides the basis for further development of novel antibacterial drugs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Alligator Sinensis

TISSUE(S): Blood Serum

SUBMITTER: Sheng-Guo Fang  

LAB HEAD: Sheng-Guo Fang

PROVIDER: PXD017090 | Pride | 2023-03-10

REPOSITORIES: Pride

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Publications

Antibacterial Properties and Potential Mechanism of Serum from Chinese Alligator.

Hu Meng-Yuan MY   Chen Yi-Wen YW   Chai Zhi-Fan ZF   Wang Yin-Zhi YZ   Lin Jian-Qing JQ   Fang Sheng-Guo SG  

Microorganisms 20221108 11


The Chinese alligator (<i>Alligator sinensis</i>) is an ancient reptile with strong immunity that lives in wetland environments. This study tested the antibacterial ability of Chinese alligator serum (CAS) against <i>Klebsiella pneumoniae</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Pseudomonas aeruginosa</i> and analyzed the potential underlying mechanisms. Results showed that the CAS had a marked antibacterial effect on <i>K. pneumoniae</i>, <i>E. coli</i>, and <i>P. aeru  ...[more]

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