Dataset Information

Hypoxia response in barley - proteomics analysis

ABSTRACT: Hypoxia stress (<1% oxygen) followed by reoxygenation, is accompanied by formation of reactive oxygen species (ROS) and nitric oxide (NO), which act as signaling molecules, but also cause nitro-oxidative stress. The NO concentration is regulated by phytoglobins (formerly hemoglobins) in the NO cycle. To understand how changes in phytoglobin expression affect plant metabolism under hypoxia, we studied the proteome and metabolome of barley (Hordeum vulgare L., cv. Golden Promise) plants without (WT) or with (HO) overexpression of phytoglobins. WT plants were more susceptible to hypoxia than HO. The chlorophyll a+b content was lowered by 50% and biomass by 30% in WT24, compared to WT, while HO plants were unaffected. We observe increase in ROS production during hypoxia treatment in WT seedlings had that was not observed in HO seedlings. But most importantly we identified and quantified 9694 proteins out of which 1107 changed significantly in abundance in at least one of the four comparisons – HO/WT andHO24/WT24) (giving genotype differences), WT24/WT and HO24/HO giving treatment differences. The main proteome changes between genotypes shown that many proteins were downregulated in HO24/HO but not in WT24/WT, such as ion transporters, Ca2+ signal transduction, and proteins related to protein degradation. We also identified and quantified 1470 metabolites, and the abundance of >500 changed significantly. Hypoxia had a much larger overall effect on the metabolome than overexpression of phytoglobin. The metabolites that have the biggest influence on the differences between treatments were selected for further analysis, among them many amino acids, putrescine and metabolites taking part in glycolysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Adelina Rogowska-Wrzesinska

LAB HEAD: Adelina Rogowska-Wrzesinska

PROVIDER: PXD017103 | Pride | 2020-03-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20181106_Barley_Hyp_1.pep.xml	Pepxml
	20191118_Barley_Hyp_1.prot.prot.xml	Xml
	Explaningrawfilessetupandnames.txt	Txt
	Mixedsampleofbiologicalreplicates1and2-fraction1.raw	Raw
	Mixedsampleofbiologicalreplicates1and2-fraction2.raw	Raw

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Publications

The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming.

Andrzejczak Olga A OA Havelund Jesper F JF Wang Wei-Qing WQ Kovalchuk Sergey S Hagensen Christina E CE Hasler-Sheetal Harald H Jensen Ole N ON Rogowska-Wrzesinska Adelina A Møller Ian Max IM Hebelstrup Kim H KH

International journal of molecular sciences 20200224 4

Overexpression of phytoglobins (formerly plant hemoglobins) increases the survival rate of plant tissues under hypoxia stress by the following two known mechanisms: (1) scavenging of nitric oxide (NO) in the phytoglobin/NO cycle and (2) mimicking ethylene priming to hypoxia when NO scavenging activates transcription factors that are regulated by levels of NO and O2 in the N-end rule pathway. To map the cellular and metabolic effects of hypoxia in barley (Hordeum vulgare L., cv. ...[more]

PMID: 32102473

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Project description:Fomitiporia mediterranea (Fmed) is one of the main fungal species found in grapevine wood rot, also called “amadou”, one of the most typical symptoms of grapevine trunk disease Esca. This fungus is functionally classified as a white-rot, able to degrade all wood structure polymers, i.e., hemicelluloses, cellulose, and the most recalcitrant component, lignin. Specific enzymes are secreted by the fungus to degrade those components, namely carbohydrate active enzymes for hemicelluloses and cellulose, which can be highly specific for given polysaccharide, and peroxidases, which enable white-rot to degrade lignin, with specificities relating to lignin composition as well. Furthermore, besides polymers, a highly diverse set of metabolites often associated with antifungal activities is found in wood, this set differing among the various wood species. Wood decayers possess the ability to detoxify these specific extractives and this ability could reflect the adaptation of these fungi to their specific environment. The aim of this study is to better understand the molecular mechanisms used by Fmed to degrade wood structure, and in particular its potential adaptation to grapevine wood. To do so, Fmed was cultivated on sawdust from different origins: grapevine, beech, and spruce. Carbon mineralization rate, mass loss, wood structure polymers contents, targeted metabolites and secreted proteins were measured. We used the well-known white-rot model Trametes versicolor for comparison. Whereas no significant degradation was observed with spruce, a higher mass loss was measured on Fmed grapevine culture compared to beech culture. Moreover, on both substrates, a simultaneous degradation pattern and the degradation of wood extractives were demonstrated, and proteomic analyses identified a relative overproduction of oxidoreductases involved in lignin and extractive degradation on grapevine cultures, and only few differences in carbohydrate active enzymes. These results could explain at least partially the adaptation of Fmed to grapevine wood structural composition compared to other wood species and suggest that other biotic and abiotic factors should be considered to fully understand the potential adaptation of Fmed to its ecological niche.

Dataset Information

Hypoxia response in barley - proteomics analysis

Dataset's files

Publications

The Hypoxic Proteome and Metabolome of Barley (<i>Hordeum vulgare</i> L.) with and without Phytoglobin Priming.

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