Dataset Information

In-depth plasma proteomics in patients with metastatic cutaneous melanoma treated with immune checkpoint inhibitors or MAPK-inhibitors

ABSTRACT: Recent development of new therapies with immune checkpoint inhibitors (ICIs) and MAPK-inhibitors (MAPKis) have significantly improved the outcome in metastatic cutaneous melanoma (CM). However, therapy response is limited to subgroups of patients and clinically useful predictive biomarkers for treatment outcome are lacking. To discover treatment-related systemic changes in plasma and potential biomarkers associated with treatment outcomes, we analysed 45 plasma samples from 24 patients with metastatic (stage IV) CM, using HiRIEF LC-MS/MS, collected before (pre-trm) and during treatment (trm). Of these, 27 samples were taken from 15 metastatic CM patients treated with ICIs (13 pre-trm and 14 trm samples; 12 matched) and 9 patients treated with MAPKis (9 matched). Matched samples were trm and pre-trm samples taken from the same individual before treatment and after the first cycle of treatment (before the second cycle). We have analysed the change in the plasma protein levels during treatment by comparing the plasma levels in the trm samples to the pre-trm samples, to detect treatment-induced alterations in the plasma proteome. We have analysed the patients treated with ICIs separately from the patients treated with MAPKis, to detect treatment-specific changes for both.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Cutaneous Malignant Melanoma 1,Melanoma,Skin Melanoma

SUBMITTER: Haris Babačić

LAB HEAD: Janne Lehtiö

PROVIDER: PXD017201 | Pride | 2020-07-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	LJMP_Melanoma_QE_20170310_TMT_set1_1mgIPG3-10_fr01.raw	Raw
	LJMP_Melanoma_QE_20170310_TMT_set1_1mgIPG3-10_fr02.raw	Raw
	LJMP_Melanoma_QE_20170310_TMT_set1_1mgIPG3-10_fr03.raw	Raw
	LJMP_Melanoma_QE_20170310_TMT_set1_1mgIPG3-10_fr04.raw	Raw
	LJMP_Melanoma_QE_20170310_TMT_set1_1mgIPG3-10_fr05.raw	Raw

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Publications

In-depth plasma proteomics reveals increase in circulating PD-1 during anti-PD-1 immunotherapy in patients with metastatic cutaneous melanoma.

Babačić Haris H Lehtiö Janne J Pico de Coaña Yago Y Pernemalm Maria M Eriksson Hanna H

Journal for immunotherapy of cancer 20200501 1

<h4>Background</h4>Immune checkpoint inhibitors (ICIs) have significantly improved the outcome in metastatic cutaneous melanoma (CM). However, therapy response is limited to subgroups of patients and clinically useful predictive biomarkers are lacking.<h4>Methods</h4>To discover treatment-related systemic changes in plasma and potential biomarkers associated with treatment outcome, we analyzed serial plasma samples from 24 patients with metastatic CM, collected before and during ICI treatment, w ...[more]

PMID: 32457125

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