Proteomics

Dataset Information

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Cyclin B1-Cdk1 binds MAD1 and facilitates its release from the nuclear pore complex to ensure a robust Spindle Assembly Checkpoint

ABSTRACT: How the cell rapidly and completely reorganises its architecture when it divides is a problem that has fascinated for almost 150 years. We now know that the core regulatory machinery is highly conserved in eukaryotes but how these multiple protein kinases, protein phosphatases, and ubiquitin ligases are coordinated in space and time to remodel the cell in a matter of minutes remains a major question. Cyclin B-Cdk is the primary kinase that drives mitotic remodelling and here we show that it is targeted to the nuclear pore complex (NPC) by binding an acidic face of the kinetochore checkpoint protein, MAD1, where it coordinates NPC disassembly with kinetochore assembly. This localised Cyclin B1-Cdk1 activity is needed for the proper release of MAD1 from the embrace of TPR at the nuclear pore so that it can be recruited to kinetochores before nuclear envelope breakdown to maintain genomic stability.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Presumptive Retinal Pigmented Epithelium, Cell Culture

SUBMITTER: James Wright  

LAB HEAD: Jyoti Choudhary

PROVIDER: PXD017202 | Pride | 2020-06-29

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
CM01_IP_8plex.msf Msf
CM01_IP_8plex.pdResult Other
CM02_IP_11plex_V1_10ppm.msf Msf
CM02_IP_11plex_V1_10ppm.pdResult Other
CM03_IP_11plex.msf Msf
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Publications

Cyclin B1-Cdk1 facilitates MAD1 release from the nuclear pore to ensure a robust spindle checkpoint.

Jackman Mark M   Marcozzi Chiara C   Barbiero Martina M   Pardo Mercedes M   Yu Lu L   Tyson Adam L AL   Choudhary Jyoti S JS   Pines Jonathon J  

The Journal of cell biology 20200601 6

How the cell rapidly and completely reorganizes its architecture when it divides is a problem that has fascinated researchers for almost 150 yr. We now know that the core regulatory machinery is highly conserved in eukaryotes, but how these multiple protein kinases, protein phosphatases, and ubiquitin ligases are coordinated in space and time to remodel the cell in a matter of minutes remains a major question. Cyclin B1-Cdk is the primary kinase that drives mitotic remodeling; here we show that  ...[more]

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