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Revisiting high resolution crystal structure of Phormidium rubidum phycocyanin using X-ray and mass spectrometry

ABSTRACT: X-ray crystolography structure deconvolution in combination with mass spectrometry sequencing

INSTRUMENT(S): Q Exactive

ORGANISM(S): Phormidium Rubidum A09dm

TISSUE(S): Photosynthetic Cell, Cell Culture

SUBMITTER: Haijun Liu

LAB HEAD: Haijun Liu

PROVIDER: PXD017229 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	20190405_PC_001.raw_Haijun.mgf	Mgf
	20190405_PC_001.raw_Haijun.mzid.gz	Mzid
	20190405_PC_001.raw_Haijun.pride.mgf.gz	Mgf
	20190405_PC_001.raw_Haijun.pride.mztab.gz	Mztab
	20190405_PC_002.raw_Haijun.mgf	Mgf

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Revisiting high-resolution crystal structure of Phormidium rubidum phycocyanin.

Sonani Ravi R RR Roszak Aleksander W AW Liu Haijun H Gross Michael L ML Blankenship Robert E RE Madamwar Datta D Cogdell Richard J RJ

Photosynthesis research 20200417 3

The crystal structure of phycocyanin (pr-PC) isolated from Phormidium rubidum A09DM (P. rubidum) is described at a resolution of 1.17 Å. Electron density maps derived from crystallographic data showed many clear differences in amino acid sequences when compared with the previously obtained gene-derived sequences. The differences were found in 57 positions (30 in α-subunit and 27 in β-subunit of pr-PC), in which all residues except one (β145Arg) are not interacting with the three phycocyanobilin ...[more]

PMID: 32303893

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