Dataset Information

Label-free Proteomics for Discovering Biomarker Candidates for Controlling Krypton Misuse in Castrated Horses (Geldings)

ABSTRACT: Recent advances in label-free quantitative proteomics may support its application in identifying and monitoring biomarkers for the purpose of doping control in equine sports. In this study, we developed a workflow of label-free quantitative proteomics to propose plasma protein biomarkers in horses after administration with krypton (Kr), a potential erythropoiesis-stimulating agent. Plasma proteomes were profiled by using nano-liquid chromatography-high resolution mass spectrometry. An in-house mass spectral library consisting of 1,121 proteins was compiled using samples collected from geldings (castrated horses) in the administration trial and geldings in training. A data-independent acquisition (DIA) method was used to quantify an array of plasma proteins across plasma samples from the administration trial. Statistical analyses proposed a profile of 83 biomarker candidates that successfully differentiated Kr-administered samples from control samples, with the ability to detect Kr exposure for up to 13 days (the last sample collected in the administration trial). The model also correctly classified 32 in-training geldings as untreated controls. This is significantly longer than the one hour detection time of plasma Kr using headspace gas chromatography-tandem mass spectrometry. Bioinformatic analyses enriched biomarker candidates relevant to complement activation and iron metabolism. The upregulation of transferrin receptor protein 1 (TFRC), one of the candidates related to iron metabolism, in plasma after Kr administration was validated by selected reaction monitoring (SRM) of corresponding peptides. These results have demonstrated label-free quantitative proteomics as a promising approach to propose plasma protein biomarkers to enhance doping control.

INSTRUMENT(S): TSQ Quantiva, Q Exactive HF

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Blood Plasma

SUBMITTER: Kin-Sing WONG

LAB HEAD: Dr Emmie N.M. Ho

PROVIDER: PXD017262 | Pride | 2020-02-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	180924_ProteomicsLibrary_v8.msf	Msf
	C1_-1B_Plasma_DIA_1.raw	Raw
	C1_0B_Plasma_DIA_1.raw	Raw
	C1_10B_Plasma_DIA_1.raw	Raw
	C1_11B_Plasma_DIA_1.raw	Raw

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Publications

Label-free Proteomics for Discovering Biomarker Candidates for Controlling Krypton Misuse in Castrated Horses (Geldings).

Wong Kin-Sing KS Cheung Hiu Wing HW Choi Timmy L S TLS Kwok Wai Him WH Curl Peter P Mechie Stewart C SC Prabhu Anil A Wan Terence S M TSM Ho Emmie N M ENM

Journal of proteome research 20200225 3

Recent advances in label-free quantitative proteomics may support its application in identifying and monitoring biomarkers for the purpose of doping control in equine sports. In this study, we developed a workflow of label-free quantitative proteomics to propose plasma protein biomarkers in horses after administration with krypton (Kr), a potential erythropoiesis-stimulating agent. Plasma proteomes were profiled by using nanoliquid chromatography-high-resolution mass spectrometry. An in-house ma ...[more]

PMID: 32050764

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