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Exposure of THP1 cells to plasma containing or lacking cfDNA

ABSTRACT: We performed all stimulatory experiments using THP1 cell line as a representative of primary human monocytes to show fundamental role of the cfDNA in healthy organisms. The experiments were conducted in duplicates using plasma containing cfDNA (NP) and the reference one with cfDNA removed by DNase (TP) to recognize unequivocally the effect of plasma cfDNA on transcriptome and proteome of monocytes. We used native human plasma samples obtained from healthy volunteers with no animal serum addition to cultivation medium in order to avoid the presence of uncharacterized animal cfDNA in the experiments.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Blood

SUBMITTER: Karel Harant

LAB HEAD: Marie Korabecna

PROVIDER: PXD017285 | Pride | 2021-05-18

REPOSITORIES: Pride

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			Action	DRS
	323-P1N.raw	Raw
	323-P1O.raw	Raw
	323-P2N_20190401162213.raw	Raw
	323-P2O.raw	Raw
	323-P3N.raw	Raw

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Publications

Cell-free DNA in plasma as an essential immune system regulator.

Korabecna M M Zinkova A A Brynychova I I Chylikova B B Prikryl P P Sedova L L Neuzil P P Seda O O

Scientific reports 20201015 1

The cell-free DNA (cfDNA) is always present in plasma, and it is biomarker of growing interest in prenatal diagnostics as well as in oncology and transplantology for therapy efficiency monitoring. But does this cfDNA have a physiological role? Here we show that cfDNA presence and clearance in plasma of healthy individuals plays an indispensable role in immune system regulation. We exposed THP1 cells to healthy individuals' plasma with (NP) and without (TP) cfDNA. In cells treated with NP, we fou ...[more]

PMID: 33060738

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