Dataset Information

Proteomic profiling of RNA binding proteins in mouse spermatogonial stem cell

ABSTRACT: RNA-binding proteins (RBPs) control the fate of RNAs. Tissue regeneration and homeostasis depend on tissue-specific stem cells, but the scope of RBP involvementin these processes remains largely unknown. Here, we identified the RBP repertoire of the mouse undifferentiated spermatogonial population that consists of stem cells and transit amplifying progenitors.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell

SUBMITTER: Tao Zhou

LAB HEAD: Xin Wu

PROVIDER: PXD017377 | Pride | 2020-03-19

REPOSITORIES: Pride

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Publications

mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation.

Du Guihua G Wang Xinrui X Luo Mengcheng M Xu Weiya W Zhou Tao T Wang Mei M Yu Luping L Li Lufan L Cai Li'e L Wang P Jeremy PJ Zhong Li John J Oatley Jon M JM Wu Xin X

Development (Cambridge, England) 20200412 8

Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA-binding proteins (RBPs) in regulation of the biological activities of this population. Proteins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking and profiled by proteomics (termed mRBPome capture), ...[more]

PMID: 32188631

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