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Comprehensive cross-linking mass spectrometry reveals molecular insights into the between peripheral and core NuRD complex subunits

ABSTRACT: Affinity purifications of CHD4 and CDK2AP2 to identify their interactors. The C-terminally tagged CHD4 has a C-terminal truncation to test whether this domain is required for the interaction with other NuRD subunits. GFP-purifications of both N- and C-terminally tagged CDK2AP2 were performed.

INSTRUMENT(S): LTQ FT, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Cornelia Spruijt

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD017378 | Pride | 2020-12-07

REPOSITORIES: Pride

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			Action	DRS
	20180329_Fusion1_SA_NS_GFP-CHD_CHD4FL_1.raw	Raw
	20180329_Fusion1_SA_NS_GFP-CHD_CHD4FL_2.raw	Raw
	20180329_Fusion1_SA_NS_GFP-CHD_CHD4FL_3.raw	Raw
	20180329_Fusion1_SA_NS_GFP-CHD_CHD4dC_1.raw	Raw
	20180329_Fusion1_SA_NS_GFP-CHD_CHD4dC_2.raw	Raw

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Comprehensive cross-linking mass spectrometry reveals molecular insights into the between peripheral and core NuRD complex subunits

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