Dataset Information

Identification of Xenopus laevis egg extract fraction stimulating somatic DNA replication through LC-MSMS

ABSTRACT: In several metazoans the number of active replication origins in embryonic nuclei is higher than in somatic ones, ensuring rapid genome duplication during synchronous embryonic cell divisions. High replication origin density can be restored by somatic nuclear reprogramming. However, mechanisms underlying high replication origin density formation coupled to rapid cell cycles are poorly understood. Here, we characterized a fraction of Xenopus egg extract able to stimulate somatic DNA replication.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Egg, Oocyte

SUBMITTER: Paolo Soffientini

LAB HEAD: Vincenzo Costanzo

PROVIDER: PXD017383 | Pride | 2020-03-19

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	F004532.dat	Other
	LF_VC_sol_200131.raw	Raw

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Publications

SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.

Falbo Lucia L Raspelli Erica E Romeo Francesco F Fiorani Simona S Pezzimenti Federica F Casagrande Francesca F Costa Ilaria I Parazzoli Dario D Costanzo Vincenzo V

Nature communications 20200312 1

In several metazoans, the number of active replication origins in embryonic nuclei is higher than in somatic ones, ensuring rapid genome duplication during synchronous embryonic cell divisions. High replication origin density can be restored by somatic nuclear reprogramming. However, mechanisms underlying high replication origin density formation coupled to rapid cell cycles are poorly understood. Here, using Xenopus laevis, we show that SSRP1 stimulates replication origin assembly on somatic ch ...[more]

PMID: 32165637

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