Dataset Information

Macrophage development and activation involve coordinated intron retention in key inflammatory regulators

ABSTRACT: Monocytes and macrophages are essential components of the innate immune system. Herein, we report that intron retention (IR) plays an important role in the development and function of these cells. Using mRNA sequencing, bioinformatics analyses and RT-qPCR validation, we identified differential IR and altered expression of key genes involved in macrophage development and function, both in vitro and in vivo. We demonstrate that decreased IR in nuclear-detained mRNA is coupled to increased expression of genes encoding regulators of macrophage transcription, phagocytosis and inflammatory signalling, including ID2, IRF7, ENG and LAT. We further show that this dynamic IR program persists during the polarisation of resting macrophages into activated macrophages. In the presence of proinflammatory stimuli, intron-retaining CXCL2 and NFKBIZ transcripts in macrophages are rapidly spliced, leading to timely expression of these key inflammatory regulators by macrophages. Our study provides novel insights into the molecular mechanisms controlling vital regulators of the innate immune response.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Mark Larance

LAB HEAD: Dr Mark Larance

PROVIDER: PXD017391 | Pride | 2020-05-27

REPOSITORIES: Pride

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			Action	DRS
	20190828_Lumos_ML_NP_EXP1_M0_Rep1.raw	Raw
	20190828_Lumos_ML_NP_EXP1_M0_Rep2.raw	Raw
	20190828_Lumos_ML_NP_EXP1_M0_Rep3.raw	Raw
	20190828_Lumos_ML_NP_EXP1_M1_48h_Rep1.raw	Raw
	20190828_Lumos_ML_NP_EXP1_M1_48h_Rep2.raw	Raw

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Macrophage development and activation involve coordinated intron retention in key inflammatory regulators.

Green Immanuel D ID Pinello Natalia N Song Renhua R Lee Quintin Q Halstead James M JM Kwok Chau-To CT Wong Alex C H ACH Nair Shalima S SS Clark Susan J SJ Roediger Ben B Schmitz Ulf U Larance Mark M Hayashi Rippei R Rasko John E J JEJ Wong Justin J-L JJ

Nucleic acids research 20200701 12

Monocytes and macrophages are essential components of the innate immune system. Herein, we report that intron retention (IR) plays an important role in the development and function of these cells. Using Illumina mRNA sequencing, Nanopore direct cDNA sequencing and proteomics analysis, we identify IR events that affect the expression of key genes/proteins involved in macrophage development and function. We demonstrate that decreased IR in nuclear-detained mRNA is coupled with increased expression ...[more]

PMID: 32449925

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