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The phosphoproteome response to dithiolthreitol in Saccharomyces cerevisiae

ABSTRACT: The project quantified the yeast phosphoproteome before and at various times after dithiolthreitol (DTT) in budding yeast, focusing on wild-type cells and mutants laking the Ire1 and Mkk1/Mkk2 kinases.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Evgenia Shishkova

LAB HEAD: Joshua J Coon

PROVIDER: PXD017396 | Pride | 2020-07-03

REPOSITORIES: pride

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			Action	DRS
	10March2016_ES_DTTstress_rep2.xlsx	Xlsx
	13June2014_ES_DDTstress_6plex_rep1_fr1_a.raw	Raw
	13June2014_ES_DDTstress_6plex_rep1_fr1_b.raw	Raw
	13June2014_ES_DDTstress_6plex_rep1_fr2_a.raw	Raw
	13June2014_ES_DDTstress_6plex_rep1_fr2_b.raw	Raw

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Publications

Phosphoproteome Response to Dithiothreitol Reveals Unique Versus Shared Features of Saccharomyces cerevisiae Stress Responses.

MacGilvray Matthew E ME Shishkova Evgenia E Place Michael M Wagner Ellen R ER Coon Joshua J JJ Gasch Audrey P AP

Journal of proteome research 20200713 8

To cope with sudden changes in the external environment, the budding yeast Saccharomyces cerevisiae orchestrates a multifaceted response that spans many levels of physiology. Several studies have interrogated the transcriptome response to endoplasmic reticulum (ER) stress and the role of regulators such as the Ire1 kinase and Hac1 transcription factors. However, less is known about responses to ER stress at other levels of physiology. Here, we used quantitative phosphoproteomics and compu ...[more]

PMID: 32597660

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