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15N labeling of mouse microbiota

ABSTRACT: This study investigated the in vivo dynamics of the microbiome using metaproteomics coupled with protein-SIP in mice.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Gut Metagenome

SUBMITTER: Xu Zhang

LAB HEAD: Daniel Figeys

PROVIDER: PXD017451 | Pride | 2021-08-26

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Mouse_184sample_2.6M.GeneSet.pep.fasta	Fasta
	Xu_20140829_15Nplight_D0_Ref1.raw	Raw
	Xu_20140829_15Nplight_D0_Ref2.raw	Raw
	Xu_20140829_15Nplight_D0_Ref3.raw	Raw
	Xu_20140829_15Nplight_D0_Ref4.raw	Raw

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Publications

Studying the Temporal Dynamics of the Gut Microbiota Using Metabolic Stable Isotope Labeling and Metaproteomics.

Smyth Patrick P Zhang Xu X Ning Zhibin Z Mayne Janice J Moore Jasmine Isabelle JI Walker Krystal K Lavallée-Adam Mathieu M Figeys Daniel D

Analytical chemistry 20201130 24

The gut microbiome and its metabolic processes are dynamic systems. Surprisingly, our understanding of gut microbiome dynamics is limited. Here, we report a metaproteomic workflow that involves protein stable isotope probing (protein-SIP) and identification/quantification of partially labeled peptides. We also developed a package, which we call MetaProfiler, that corrects for false identifications and performs phylogenetic and time series analysis for the study of microbiome dynamics. From the s ...[more]

PMID: 33253538

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Sequence database choice significantly affects taxonomic and functional metaproteomic results in gut microbiota studies

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