Proteomics

Dataset Information

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Dynamic complexome profiling identifies impaired protein turnover in mouse CLPP-/-embryonic fibroblasts

ABSTRACT: We studied dynamics within mitochondrial complexes in MEFs from CLPP-/- and wildtype mice.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell

SUBMITTER: Ilka Wittig  

LAB HEAD: Aleksandra Trifunovic

PROVIDER: PXD017463 | Pride | 2020-03-05

REPOSITORIES: Pride

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Dataset's files

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Complexome_5hours_SILAC.xlsx Xlsx
Complexome_5hr.jpg Other
P18_093_Turnover_with_Replicates_5h_KO2_01.raw Raw
P18_093_Turnover_with_Replicates_5h_KO2_02.raw Raw
P18_093_Turnover_with_Replicates_5h_KO2_03.raw Raw
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Publications

Regulation of the turnover of complex I (CI), the largest mitochondrial respiratory chain complex, remains enigmatic despite huge advancement in understanding its structure and the assembly. Here, we report that the NADH-oxidizing N-module of CI is turned over at a higher rate and largely independently of the rest of the complex by mitochondrial matrix protease ClpXP, which selectively removes and degrades damaged subunits. The observed mechanism seems to be a safeguard against the accumulation  ...[more]

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