Dataset Information
Dynamic complexome profiling identifies impaired protein turnover in mouse CLPP-/-embryonic fibroblasts
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Primary Cell
SUBMITTER: 
Ilka Wittig
LAB HEAD: Aleksandra Trifunovic
PROVIDER: PXD017463 | Pride | 2020-03-05
REPOSITORIES: Pride
Publications
A salvage pathway maintains highly functional respiratory complex I.
Nature communications 20200402 1
Regulation of the turnover of complex I (CI), the largest mitochondrial respiratory chain complex, remains enigmatic despite huge advancement in understanding its structure and the assembly. Here, we report that the NADH-oxidizing N-module of CI is turned over at a higher rate and largely independently of the rest of the complex by mitochondrial matrix protease ClpXP, which selectively removes and degrades damaged subunits. The observed mechanism seems to be a safeguard against the accumulation ...[more]