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Autofluorescence positive and negative microglia constitute novel subsets found in healthy brain.

ABSTRACT: Utilizing autofluorescence (AF) as a discriminating parameter, we identified two novel microglia subsets in both mice, termed autofluorescence-positive (AF+) and negative (AF-), and characterized them by label-free proteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Dirk Walther

LAB HEAD: Dirk Walther

PROVIDER: PXD017505 | Pride | 2020-06-26

REPOSITORIES: Pride

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			Action	DRS
	OL1_191219_DW1_S_MicrogliaPopulations_AFNegative-1.raw	Raw
	OL1_191219_DW1_S_MicrogliaPopulations_AFNegative-2.raw	Raw
	OL1_191219_DW1_S_MicrogliaPopulations_AFNegative-3.raw	Raw
	OL1_191219_DW1_S_MicrogliaPopulations_AFNegative-4.raw	Raw
	OL1_191219_DW1_S_MicrogliaPopulations_AFNegative-5.raw	Raw

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Publications

Differential accumulation of storage bodies with aging defines discrete subsets of microglia in the healthy brain.

Burns Jeremy Carlos JC Cotleur Bunny B Walther Dirk M DM Bajrami Bekim B Rubino Stephen J SJ Wei Ru R Franchimont Nathalie N Cotman Susan L SL Ransohoff Richard M RM Mingueneau Michael M

eLife 20200624

To date, microglia subsets in the healthy CNS have not been identified. Utilizing autofluorescence (AF) as a discriminating parameter, we identified two novel microglia subsets in both mice and non-human primates, termed autofluorescence-positive (AF+) and negative (AF-). While their proportion remained constant throughout most adult life, the AF signal linearly and specifically increased in AF+ microglia with age and correlated with a commensurate increase in si ...[more]

PMID: 32579115

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Brain injury accelerates the onset of a reversible age-related microglial phenotype associated with hyperphagocytosis and inflammatory neurodegeneration

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