Dataset Information

Hunting monolignol transporter(s): membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce

ABSTRACT: Monolignol transport during lignification is a partially solved puzzle: both the mechanism(s) and the transported form of monolignols are unknown in developing xylem of trees. We tested a hypothesis of an active, plasma membrane (PM)-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared of developing xylem, phloem, and lignin-forming, tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as of a control material, non-lignifying tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested this transport being over the tonoplast. Based on similar inhibitor assays, lignin-forming, tissue42 cultured cells of spruce had coniferin transport putatively localizing on PM. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis of membrane proteins isolated from spruce developing xylem, phloem and tissue-cultured cells revealed multiple transporters. These were compared to a transporter gene set that was gained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for the ABC-transporter-mediated monolignol transport but point to secondary active transporters (such as MFS- or MATE-transporters). In contrast, proteomic and co-expression analyses suggest a role for ABC-transporters and MFS-transporters.

INSTRUMENT(S): Synapt G2-S HDMS

ORGANISM(S): Picea Abies

TISSUE(S): Wood

DISEASE(S): Not Available

SUBMITTER: Joakim Bygdell

LAB HEAD: Anna Kärkönen

PROVIDER: PXD017533 | Pride | 2020-08-14

REPOSITORIES: Pride

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Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce.

Väisänen Enni E Takahashi Junko J Obudulu Ogonna O Bygdell Joakim J Karhunen Pirkko P Blokhina Olga O Laitinen Teresa T Teeri Teemu H TH Wingsle Gunnar G Fagerstedt Kurt V KV Kärkönen Anna A

Journal of experimental botany 20201001 20

Both the mechanisms of monolignol transport and the transported form of monolignols in developing xylem of trees are unknown. We tested the hypothesis of an active, plasma membrane-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from developing xylem and lignin-forming tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as from control materials, comprising non-lignifying Norway spruce phloem and tobacco (Nicotiana t ...[more]

PMID: 32777074

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