Proteomics

Dataset Information

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MicroRNA-host gene lncRNA regulates the cell cycle re-entry post cellular quiescence


ABSTRACT: Cellular quiescence is coupled with cellular development, tissue homeostasis, and cancer progression. Both quiescence and cell cycle re-entry are controlled by active and precise regulation of gene expression. However, the roles of long noncoding RNAs (lncRNAs) during these processes remain to be elucidated. By performing a genome-wide transcriptome analyses, we identify thousands of differentially expressed lncRNAs, including ~30 of the less-characterized class of microRNA-host-gene lncRNAs (lnc-MIRHGs), during cellular quiescence and during serum-stimulation in human diploid cells. We observe that the mature MIR222HG display serum-stimulated induction due to enhanced pre-RNA splicing. Serum-stimulated binding of the pre-mRNA splicing factor SRSF1 to a micro-exon, which partially overlaps with the primary miR-222 precursor, facilitates enhanced MIR222HG splicing. In serum-stimulated cells, SRSF1 negatively regulates the Drosha/DGCR8-catalyzed cleavage of pri-miR-222, thereby increasing the cellular pool of the mature MIR222HG. Further, loss-of-function studies indicate that the mature MIR222HG facilitates the serum-stimulated cell cycle re-entry in a microRNA-independent manner. Mechanistically, MIR222HG, along with ILF3/2 complex, forms RNA:RNA duplex with DNM3OS lncRNA, thereby promoting DNM3OS stability. The current study identifies a mechanism in which the interplay between splicing versus microprocessor complex dictates the serum-induced expression of lnc-MIRHG MIR222HG for efficient cell cycle re-entry.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast

SUBMITTER: Lisa Jenkins  

LAB HEAD: Lisa M. Jenkins

PROVIDER: PXD017585 | Pride | 2020-09-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
222_1.mzid.gz Mzid
222_2.mzid.gz Mzid
222_3.mzid.gz Mzid
YFP1.mzid.gz Mzid
YFP2.mzid.gz Mzid
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Publications

Antagonism between splicing and microprocessor complex dictates the serum-induced processing of lnc-<i>MIRHG</i> for efficient cell cycle reentry.

Sun Qinyu Q   Hao Qinyu Q   Lin Yo-Chuen YC   Song You Jin YJ   Bangru Sushant S   Arif Waqar W   Tripathi Vidisha V   Zhang Yang Y   Cho Jung-Hyun JH   Freier Susan M SM   Jenkins Lisa M LM   Ma Jian J   Yoon Je-Hyun JH   Kalsotra Auinash A   Lal Ashish A   Prasanth Supriya G SG   Prasanth Kannanganattu V KV  

RNA (New York, N.Y.) 20200716 11


Cellular quiescence and cell cycle reentry regulate vital biological processes such as cellular development and tissue homeostasis and are controlled by precise regulation of gene expression. The roles of long noncoding RNAs (lncRNAs) during these processes remain to be elucidated. By performing genome-wide transcriptome analyses, we identify differential expression of several hundreds of lncRNAs, including a significant number of the less-characterized class of microRNA-host-gene (<i>MIRHG</i>)  ...[more]

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