Dataset Information

Comparative proteomics of three Giardia lamblia strains: Antigenic variation in the post-genomic era

ABSTRACT: Giardia lamblia is a causative agent of persistent diarrhea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identified 398 proteins differentially expressed between the GS and both WB isolates and 97 proteins differentially expressed between both WB isolates. We investigated the expression levels of the predominant variant-specific surface proteins (VSPs) in each clone and matched the previously described major VSPs of each strain to the corresponding open reading frame (ORF) sequences identified by whole-genome sequencing efforts. Furthermore, since the original WB isolate comes from a patient treated with metronidazole, we compared the susceptibilities of the strains to nitro compounds, as well the expression levels of enzymes involved in nitro reduction and on the corresponding enzyme activities and found distinct differences between the three strains.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Giardia Intestinalis (giardia Lamblia)

TISSUE(S): Cell Culture

SUBMITTER: Manfred Heller

LAB HEAD: Loachim Müller

PROVIDER: PXD017597 | Pride | 2020-05-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20190212_2_WBC6_JM_i01.raw	Raw
	20190212_2_WBC6_JM_i02.raw	Raw
	20190212_2_WBC6_JM_i03.raw	Raw
	20190212_6_A1_JM_i01.raw	Raw
	20190212_6_A1_JM_i02.raw	Raw

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Comparative proteomics of three <i>Giardia lamblia</i> strains: investigation of antigenic variation in the post-genomic era.

Müller Joachim J Braga Sophie S Uldry Anne-Christine AC Heller Manfred M Müller Norbert N

Parasitology 20200427 9

Giardia lamblia is a causative agent of persistent diarrhoea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well-characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identifie ...[more]

PMID: 32338227

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