Proteomics

Dataset Information

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SWATH proteome for the coral reef fish, Acanthochromis polyacanthus, under ocean acidification and ocean warming


ABSTRACT: Construction of a comprehensive spectral library for the coral reef fish, Acanthochromis polyacanthus, from both DIA and DDA MS runs. The spectral library was then used to quantify proteomes of individual fish exposed to different environmental conditions including ocean acidification and ocean warming. Proteomes were measured for both liver and brain tissue and differential expression between environmental conditions was analyzed.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Acanthochromis Polyacanthus

TISSUE(S): Brain, Liver

SUBMITTER: Alison Monroe  

LAB HEAD: Timothy Ravasi

PROVIDER: PXD017605 | Pride | 2020-03-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20180420_Fs_DIA_10B.raw Raw
20180420_Fs_DIA_11B.raw Raw
20180420_Fs_DIA_12B.raw Raw
20180420_Fs_DIA_13B.raw Raw
20180420_Fs_DIA_14B.raw Raw
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Publications

Probing SWATH-MS as a tool for proteome level quantification in a nonmodel fish.

Monroe Alison A AA   Zhang Huoming H   Schunter Celia C   Ravasi Timothy T  

Molecular ecology resources 20200804 6


Quantitative proteomics via mass spectrometry can provide valuable insight into molecular and phenotypic characteristics of a living system. Recent mass spectrometry developments include data-independent acquisition (SWATH/DIA-MS), an accurate, sensitive and reproducible method for analysing the whole proteome. The main requirement for this method is the creation of a comprehensive spectral library. New technologies have emerged producing larger and more accurate species-specific libraries leadi  ...[more]

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