Dataset Information

Identification of phosphorylation sites in the IGF-1 Receptor

ABSTRACT: Although Insulin-like Growth Factor (IGF-1) signaling promotes tumor growth and cancer progression, IGF-1 Receptor-targeted therapies have shown poor clinical efficacy. The mechanistic basis for this is unclear as is our understanding of what distinguishes IGF-1R signaling from the closely related Insulin receptor (IR) signaling. This study illuminates both issues. A site in the IGF-1R C-terminal tail incorporating two tyrosines that are not present in the Insulin receptor (IR) was previously shown to be essential for IGF-1-mediated cancer cell survival, migration and tumorigenic growth. Here, we establish that the Y1250/Y1251 site is autophosphorylated in a cell adhesion-dependent manner.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Rosemary O'Connor

LAB HEAD: Rosemary O'Connor

PROVIDER: PXD017644 | Pride | 2020-05-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	GK-ROC-1-LocalDB-120201.htm	Other
	GK-ROC-2-LocalDB-120201.htm	Other
	GK-ROC-3-LocalDB-120201.htm	Other
	GeraldineKelly-077_2012.pptx	Other
	ROC-igfTrypAspNLocalPeptide.xlsx	Xlsx

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Publications

IGF-1 receptor activity in the Golgi of migratory cancer cells depends on adhesion-dependent phosphorylation of Tyr<sup>1250</sup> and Tyr<sup>1251</sup>.

Rieger Leonie L O'Shea Sandra S Godsmark Grant G Stanicka Joanna J Kelly Geraldine G O'Connor Rosemary R

Science signaling 20200526 633

Although insulin-like growth factor 1 (IGF-1) signaling promotes tumor growth and cancer progression, therapies that target the IGF-1 receptor (IGF-1R) have shown poor clinical efficacy. To address IGF-1R activity in cancer cells and how it differs from that of the closely related insulin receptor (IR), we focused on two tyrosines in the IGF-1R C-terminal tail that are not present in the IR and are essential for IGF-1-mediated cancer cell survival, migration, and tumorigenic growth. We found tha ...[more]

PMID: 32457113

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