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Testis proteome of Trypanosoma cruzi infected mice

ABSTRACT: Male CD-1 mice (N=8) were intraperitoneally infected by culture-derived Trypanosoma cruzi trypomastigotes of the Brazil strain. One group (N=4) was treated with an antitrypanosomal drug (nifurtimox) on day 74 post-infection (chronic phase) for 20 days. A group of uninfected mice (N=3) was also included as control. All groups (N=11) were enthanized on day 110 post-infection, testes were collected and subjected to 2D-LC-MS/MS analysis. Label-free quantitation revealed that the testes of T. cruzi-infected mice had an altered proteome pattern relative to treated mice and uninfected controls. The analysis also demonstrated that the left and right testes had different proteomic profiles in infected animals.

INSTRUMENT(S): maXis

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

DISEASE(S): Trypanosomiasis

SUBMITTER: Momar Ndao

LAB HEAD: Momar Ndao

PROVIDER: PXD017802 | Pride | 2023-03-10

REPOSITORIES: Pride

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			Action	DRS
	A1L-frac01.mgf	Mgf
	A1L-frac02.mgf	Mgf
	A1L-frac03.mgf	Mgf
	A1L-frac04.mgf	Mgf
	A1L-frac05.mgf	Mgf

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Publications

New metabolic signature for Chagas disease reveals sex steroid perturbation in humans and mice.

Golizeh Makan M Nam John J Chatelain Eric E Jackson Yves Y Ohlund Leanne B LB Rasoolizadeh Asieh A Camargo Fabio Vasquez FV Mahrouche Louiza L Furtos Alexandra A Sleno Lekha L Ndao Momar M

Heliyon 20221215 12

The causative agent of Chagas disease (CD), <i>Trypanosoma cruzi</i>, claims thousands of lives each year. Current diagnostic tools are insufficient to ensure parasitological detection in chronically infected patients has been achieved. A host-derived metabolic signature able to distinguish CD patients from uninfected individuals and assess antiparasitic treatment efficiency is introduced. Serum samples were collected from chronic CD patients, prior to and three years after treatment, and subjec ...[more]

PMID: 36590505

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