Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Peptidomics of sorted enteroendocrine cells from human organoids

ABSTRACT: A human organoid culture system was set up to grow enteroendocrine cells with a venus labeled on the glucagon gene promoter sequence. This enabled the sorting of glucagon gene positive cells from negative cells, thereby enabling the enrichment of glucagon producing cells for study. Both Venus positive and venus negative cell populations were collected and their peptidome was assessed using nano LC-MS/MS

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Small Intestine

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Richard Kay

LAB HEAD: Fiona Gribble

PROVIDER: PXD017825 | Pride | 2020-07-01

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20022102.mgf	Mgf
	20022102.pride.mgf.gz	Mgf
	20022102.raw	Raw
	20022104.mgf	Mgf
	20022104.pride.mgf.gz	Mgf

Items per page:

1 - 5 of 23

Similar Datasets

Mouse duodenal enteroendocrine transcriptome

Project description:Using genetically modifed mice to express fluorescent reporter in proglucagon expressing cells (GluVenus) or in all enteroendocrine cells (NeuroD1), we purifed positive and negative cells by FACS from duodenal epithelial cells and identified transcripts enriched in positive populations

2018-12-31 | GSE114913 | GEO

Implement enhanced artificial “intestinal enteroendocrine L cells” via oral administration for effective treatment of type 2 diabetes

Project description:Intestinal enteroendocrine L cells, responsive for secreting glucagon-like peptide-1 (GLP-1) to normalize blood glucose level, are negatively affected when type 2 diabetes (T2D) develops. Here, we designed enhanced artificial “intestinal enteroendocrine L cells” (EcN-GLP-1@Fe-TA-Pe@CLGN) for T2D treatment via oral administration.

2024-08-09 | PXD054739 |

ID2 controls differentiation of entero-endocrine cells in mouse small intestine

Project description:Aim: The mammalian gut is the largest endocrine organ. Dozens of hormones secreted by enteroendocrine cells regulate a variety of physiological functions of the gut but also of the pancreas and brain. Here, we examined the role of the helix-loop-helix transcription factor ID2 during the differentiation of intestinal stem cells along the enteroendocrine lineage. Methods: To assess the functions of ID2 in the adult mouse small intestine, we used single-cell RNA sequencing, genetically modified mice, and organoid assays. Results: We found that in the adult intestinal epithelium Id2 is predominantly expressed in enterochromaffin and peptidergic enteroendocrine cells. Consistently, the loss of Id2 leads to the reduction of Chromogranin A-positive enteroendocrine cells. In contrast, the numbers of tuft cells are increased in Id2 mutant small intestine. Moreover, ablation of Id2 elevates the numbers of Serotonin+ enterochromaffin cells and Ghrelin+ X-cells in the posterior part of the small intestine. Finally, Id2 acts downstream of BMP signalling during the differentiation of Glucagon Like Peptide-1+ L-cells and Cholecystokinin+ I-cells towards Neurotensin+PYY+ N-cells. Conclusion: Id2 plays an important role in cell fate decisions in the adult small intestine. Firstly, ID2 suppresses the differentiation of secretory intestinal epithelial progenitors towards tuft cell lineage and thus controls host immune response on commensal and parasitic microbiota. Next, ID2 is essential for establishing a differentiation gradient for enterochromaffin and X-cells along the anterior-posterior axis of the gut. Finally, ID2 is necessary for the differentiation of N-cells thus ensuring a differentiation gradient along the crypt-villi axis.

2022-06-21 | GSE178586 | GEO

An integral atlas of hormone peptides of human EECs and their dedicated proteases

Project description:Enteroendocrine cells (EECs) secrete hormones in response to ingested nutrients to control physiological processes such as appetite and insulin release. EEC hormones are synthesized as large pre-proteins that undergo proteolytic processing through peptidases to generate bioactive peptides. Due to the rarity of EECs and lack of cell models, prohormone processing remains challenging to assess. Here, we generated a panel of human organoid models in which EECs can efficiently be induced, combined with CRISPR-Cas9-mediated genetic inactivation or alteration of peptidases. We employed mass spectrometry based analysis to monitor peptide processing in knock-outs and wild type organoids for various hormone-processing enzymes (including PCSK1, PCSK2, PCSK1N, PCSK1mut_S375G, PCSK2mut_R430W, DPP4, CPB1 and CPE). We identify PCSK2-dependent Glucagon production in EECs, which is stimulated by activation of BMP signaling. We further map the substrates of other major EECs endo- and exopeptidases.

2022-11-26 | PXD033369 | Pride

Project description:Hob6 positive and negative cells

| PRJNA193190 | ENA

Peptidomics of enteroendocrine cells and characterisation of potential effects of a novel preprogastrin derived-peptide on glucose tolerance in lean mice

Project description:To analyse the peptidomics of mouse enteroendocrine cells (EECs) and human gastrointestinal (GI) tissue and identify novel gut derived peptides. High resolution nano-flow liquid chromatography mass spectrometry (LCMS) was performed on (i) flow-cytometry purified NeuroD1 positive cells from mouse and homogenised human intestinal biopsies, (ii) supernatants from primary murine intestinal cultures, (iii) intestinal homogenates from mice fed high fat diet. Candidate bioactive peptides were selected on the basis of species conservation, high expression/biosynthesis in EECs and evidence of regulated secretion in vitro. Candidate novel gut-derived peptides were chronically administered to mice to assess effects on food intake and glucose tolerance.

2021-03-29 | PXD024050 | Pride

RNA-Seq analysis of mouse small intestine enteroendocrine cells

Project description:We generated knock-in mice expressing GFP under the control of the endogenous GIP (Glucose-dependent Insulinotropic Polypeptide) promoter that enable the isolation of a purified population of small intestine K cells. Using RNA-Seq, we comprehensively characterized the transcriptomes of GIP-GFP cells as well as the entire enteroendocrine lineage derived from Neurogenin3 (Ngn3)-expressing progenitors. We interrogated the whole transcriptome of FACS-isolated small intestine GIPGFP cells using high-throughput mRNA sequencing. We also obtained the global gene expression patterns of the entire enteroendocrine cell lineage as well as the non-enteroendocrine cell population, comprising enterocytes, goblet cells and Paneth cells. To achieve this, small intestine epithelial cells from male mice resulting from the breeding of Neurogenin3 (Ngn3)-Cre mice with ROSA26-LoxP-STOP-LoxP-tomato indicator mice were isolated based on Tomato fluorescence and negative staining for CD45. Due to the small cell numbers, we constructed each of the three RNA-Seq libraries (GIPGFP, Ngn3TOMATO, and Ngn3-) using a pool of equal amounts of individual RNA samples without RNA amplification.

2015-01-01 | E-GEOD-61250 | biostudies-arrayexpress

Single-cell RNA sequencing of normal, early glioblastoma, and late glioblastoma flow sorted by CD45

Project description:Genetically engineered mouse glioblastoma tumors and normal cells at different stages were flow cytometry sorted into CD45 positive and CD45 negative populations followed by single cell RNA seq.

2022-04-06 | GSE196175 | GEO

microRNA profile analysis of sorted cells during different reprogramming stages

Project description:Mouse embryonic fibroblasts were reprogrammed using Oct4, Sox2, Klf4 and cMyc genes. At day 5, cells were sorted as Thy1 positive and Thy1 negative populations. microRNA expression profile from Thy1+ and Thy1- cells was compared with original MEFs (D0) to identify significantly changed microRNAs during initial stage of reprogramming

2015-09-12 | E-MTAB-2932 | biostudies-arrayexpress

Hippocampal cells multiplex positive and negative engrams

Project description:Hippocampal cells multiplex positive and negative engrams

| PRJNA858461 | ENA