Proteomics

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LC-MS/MS identification of interactors of the plant natriuretic peptide, AtPNP-A

ABSTRACT: Plant natriuretic peptides (PNPs) comprise a class of systemically mobile molecules functioning as hormonal regulators of ion and fluid balance. Since the molecular mode of action of PNPs remains unclear, a large-scale protein-protein interaction study has been undertaken to identify interactors of the model PNP present in Arabidopsis thaliana, termed AtPNP-A.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Mesophyll Cell

SUBMITTER: Ilona Turek  

LAB HEAD: Chris Gehring

PROVIDER: PXD017925 | Pride | 2020-04-07

REPOSITORIES: Pride

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A_thaliana_CL_AC_MS20121020_term_23 (F002294).mzid_A_thaliana_CL_AC_MS20121020_term_23_(F002294).pride.mgf.gz Mzid
A_thaliana_CL_AC_MS20121020_term_23F002294.mzid_A_thaliana_CL_AC_MS20121020_term_23_F002294.MGF Mzid
A_thaliana_CL_AC_MS20121020_term_30 (F002302).mzid_A_thaliana_CL_AC_MS20121020_term_30_(F002302).pride.mgf.gz Mzid
A_thaliana_CL_AC_MS20121020_term_30F002302.mzid_A_thaliana_CL_AC_MS20121020_term_30_F002302.MGF Mzid
A_thaliana_CL_AC_MS20121020_term_35 (F002308).mzid_A_thaliana_CL_AC_MS20121020_term_35_(F002308).pride.mgf.gz Mzid
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Dataset on interactors of the <i>Arabidopsis thaliana</i> Plant Natriuretic Peptide (AtPNP-A) determined by mass spectrometry.

Turek Ilona I   Irving Helen H   Gehring Chris C  

Data in brief 20200422

Interactors of the plant natriuretic peptide present in <i>Arabidopsis thaliana</i>, termed AtPNP-A, were affinity-based isolated from <i>A. thaliana</i> (Col-0) leaf mesophyll cell protoplasts by incubating the protoplasts with biologically active biotinylated peptide corresponding to amino acid sequence of the active site of AtPNP-A (pAtPNP-A), either in the presence or absence of a cross-linking agent, 3,3'-dithiobis(sulfosuccinimidyl propionate) (DTSSP), or with equimolar amount of biotin wi  ...[more]

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