Proteomics

Dataset Information

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Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion


ABSTRACT: The long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Sl1344

SUBMITTER: Christoph Krisp  

LAB HEAD: Marc Erhardt

PROVIDER: PXD017935 | Pride | 2020-04-27

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
160712_PS_FliB1.raw Raw
160712_PS_FliB2.raw Raw
160712_PS_FliB3.raw Raw
160712_PS_FliB_delta1.raw Raw
160712_PS_FliB_delta2.raw Raw
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Publications


The long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroe  ...[more]

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