Dataset Information

Abiotic drivers of protein abundance variation among natural populations

ABSTRACT: Identifying when and where environmental change induces molecular responses in natural populations is an important goal in contemporary ecology as it can aid in identifying molecular signatures of populations experiencing stressful conditions and potentially inform if species are approaching the limits of their tolerance niches. Achieving this goal is hampered by our understanding of the influence of environmental variation on the molecular systems of most ecologically relevant species as the pathways underlying fitness-affecting plastic responses have primarily been studied in model organisms under controlled laboratory conditions. To start overcoming this limitation, we establish relationships between protein abundance patterns and the abiotic environment. Profiling the proteomes of 24 natural populations of the putatively cold-adapted species Crunoecia irrorata and subsequently relating these profiles to natural variations in their abiotic freshwater spring habitats shows that protein abundances and networks respond to variation according to the functional roles these proteins have. We provide evidence that geographic and environmental distances affect protein abundances and identifications and that baseline abundances can be determined and used as information rather than noise in comparative field studies. Taking this naturally induced variation into account is a prerequisite if we are to identify the effects environmental change has on natural populations.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Crunoecia Irrorata

TISSUE(S): Whole Body

SUBMITTER: Joshua Ebner

LAB HEAD: Joshua Niklas Ebner

PROVIDER: PXD017959 | Pride | 2020-10-05

REPOSITORIES: Pride

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Abiotic and past climatic conditions drive protein abundance variation among natural populations of the caddisfly Crunoecia irrorata.

Ebner Joshua Niklas JN Ritz Danilo D von Fumetti Stefanie S

Scientific reports 20200923 1

Deducing impacts of environmental change on species and the populations they form in nature is an important goal in contemporary ecology. Achieving this goal is hampered by our limited understanding of the influence of naturally occurring environmental variation on the molecular systems of ecologically relevant species, as the pathways underlying fitness-affecting plastic responses have primarily been studied in model organisms and under controlled laboratory conditions. Here, to test the hypoth ...[more]

PMID: 32968134

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Project description:As a result of increasing thermal fluctuations and mean temperature values, organisms will experience conditions beyond their physiological limits. In situ adaptation to thermal regimes is mediated via directional selection and phenotypic plasticity. The latter involves physiological and morphological adjustments realized by underlying molecular mechanisms. Understanding species' adaptive capacities requires investigating these adjustive processes. Yet, acclimation through phenotypic plasticity remains largely unexplored, especially at the molecular level; For example, whether cold-adapted species inhabiting freshwater spring ecosystems have evolved adaptive mechanisms to cope with warming of freshwater habitats has, to our knowledge, never been investigated. This work reports a comprehensive proteomics study of the stenotopic species Crunoecia irrorata (Curtis, 1834) (Trichoptera: Lepistomatidae) acclimated to 10, 15 and 20 °C for 168 h. A liquid chromatography tandem mass spectrometry (LC-MS/MS)-based shotgun proteomics approach identified molecular mechanisms underlying acclimation. We constructed a homology-based database by combining genomic and transcriptomic data from related species and quantified 1356 proteins, of which 186 were differentially expressed between temperature treatments. Through functional annotation, we identified candidate proteins facilitating, among others, trehalose accumulation, tracheal system alteration, and heat shock protein regulation, then discuss concomitant ecophysiological implications. These results provide new insights into the mechanisms of adaptive responses to warming of species inhabiting freshwater ecosystems sensitive to climate change. Further, identified candidate proteins will aid in developing targeted experiments to understand their compensatory physiology. To our knowledge, this is the first study utilizing this approach to investigate the nature of phenotypic plasticity of aquatic macroinvertebrates.

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