Proteomics

Dataset Information

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Changes in golden Syrian hamster HDL proteome caused by altered cholesteryl ester transfer protein expression


ABSTRACT: Cholesteryl ester transfer protein (CETP) transfers cholesteryl ester (CE) and triglyceride (TG) between lipoproteins, which alters lipoprotein metabolism. Hamster and human CETPs have very different preferences for CE versus TG as substrate. To assess the impact of altering CETP’s substrate preference on lipoproteins in vivo, human CETP was expressed in hamsters (Mesocricetus auratus). Chow-fed hamsters received adenoviruses expressing no CETP (Ad-Null), hamster CETP (Ad-hamster CETP) or human CETP (Ad-human CETP). High density lipoproteins were isolated from hamster plasma 6 days after adenovirus injection by ultracentrifugation as the 1.063 - 1.21 g/ml density fraction. HDL proteins were precipitated with cold acetone and subjected to LC+MS/MS analysis

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mesocricetus Auratus (golden Hamster)

TISSUE(S): Blood Plasma

SUBMITTER: Ling Li  

LAB HEAD: Richard Morton

PROVIDER: PXD017998 | Pride | 2020-06-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Ad-Hamster-CETP-1.mzML Mzml
Ad-Hamster-CETP-1.mzid.gz Mzid
Ad-Hamster-CETP-1.pride.mgf.gz Mgf
Ad-Hamster-CETP-1.pride.mztab.gz Mztab
Ad-Hamster-CETP-1.raw Raw
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Publications

The lipid transfer properties of CETP define the concentration and composition of plasma lipoproteins.

Morton Richard E RE   Liu Yan Y  

Journal of lipid research 20200626 8


Cholesteryl ester transfer protein (CETP) facilitates the net transfer of cholesteryl esters (CEs) and TGs between lipoproteins, impacting the metabolic fate of these lipoproteins. Previous studies have shown that a CETP antibody can alter CETP's preference for CE versus TG as transfer substrate, suggesting that CETP substrate preference can be manipulated in vivo. Hamster and human CETPs have very different preferences for CE and TG. To assess the effect of altering CETP's substrate preference  ...[more]

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