Dataset Information

Epitope shaving and fungal immune evasion – cell wall proteomes

ABSTRACT: The cell wall provides a major physical interface between fungal pathogens and their mammalian host. This extracellular armour is critical for fungal cell homeostasis and survival. Yet essential cell wall moieties, such as β-1,3-glucan, are recognised as pathogen-associated molecular patterns (PAMPs) that activate immune-mediated clearance mechanisms. We have reported that the opportunistic human fungal pathogen, Candida albicans, masks β-1,3-glucan following exposure to lactate, hypoxia or iron depletion. However, the precise mechanism(s) by which C. albicans masks β-1,3-glucan have remained obscure. Here, we performed proteomic analysis of cell walls from C. albicans cells grown in hypoxia or lactate compared to glucose-grown controls to identify mechanisms driving β-1,3-glucan masking.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candida Albicans (yeast)

TISSUE(S): Cell Culture

SUBMITTER: David Stead

LAB HEAD: Professor Alistair J P Brown

PROVIDER: PXD018027 | Pride | 2020-06-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CGD_SC5314_A22_20151122.fasta	Fasta
	WT_GluLac_Cell_Wall_1.msf	Msf
	WT_GluLac_Cell_Wall_1.pep.xml	Pepxml
	WT_GluLac_Cell_Wall_1.raw	Raw
	WT_GluLac_Cell_Wall_2.msf	Msf

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Publications

Epitope Shaving Promotes Fungal Immune Evasion.

Childers Delma S DS Avelar Gabriela Mol GM Bain Judith M JM Pradhan Arnab A Larcombe Daniel E DE Netea Mihai G MG Erwig Lars P LP Gow Neil A R NAR Brown Alistair J P AJP

mBio 20200707 4

The cell wall provides a major physical interface between fungal pathogens and their mammalian host. This extracellular armor is critical for fungal cell homeostasis and survival. Fungus-specific cell wall moieties, such as β-1,3-glucan, are recognized as pathogen-associated molecular patterns (PAMPs) that activate immune-mediated clearance mechanisms. We have reported that the opportunistic human fungal pathogen <i>Candida albicans</i> masks β-1,3-glucan following exposure to lactate, hypoxia, ...[more]

PMID: 32636248

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