Dataset Information

The interactome of BTN3A1 (Butyrophilin Subfamily 3 Member A1)

ABSTRACT: In this study we dissected the BTN3A1 interactome before or after the treatment with pamidronate to gain mehcanistic insights into the gamma9/delta2 T cell dependent recognition of target cells. Our approach relied on affinity purification couple with mass spectrometry from a BTN3A knock-out cell line stably reconstituted with FLAG-BTN3A1. Our findings revealed an important role of cellular trafficking of BTN3A1 in the gamma9/delta2 TCR-induced T cell activation.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Domenico Fasci

LAB HEAD: Albert J. R. Heck

PROVIDER: PXD018055 | Pride | 2020-06-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20191129_OR16_Ag6_fasci001_SA_FA1_PAM_flagIP_1.raw	Raw
	20191129_OR16_Ag6_fasci001_SA_FA1_PAM_flagIP_2.raw	Raw
	20191129_OR16_Ag6_fasci001_SA_FA1_PAM_flagIP_3.raw	Raw
	20191129_OR16_Ag6_fasci001_SA_FA1_flagIP_1.raw	Raw
	20191129_OR16_Ag6_fasci001_SA_FA1_flagIP_2.raw	Raw

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Publications

γ9δ2T cell diversity and the receptor interface with tumor cells.

Vyborova Anna A Beringer Dennis X DX Fasci Domenico D Karaiskaki Froso F van Diest Eline E Kramer Lovro L de Haas Aram A Sanders Jasper J Janssen Anke A Straetemans Trudy T Olive Daniel D Leusen Jeanette J Boutin Lola L Nedellec Steven S Schwartz Samantha L SL Wester Michael J MJ Lidke Keith A KA Scotet Emmanuel E Lidke Diane S DS Heck Albert Jr AJ Sebestyen Zsolt Z Kuball Jürgen J

The Journal of clinical investigation 20200901 9

γ9δ2T cells play a major role in cancer immune surveillance, yet the clinical translation of their in vitro promise remains challenging. To address limitations of previous clinical attempts using expanded γ9δ2T cells, we explored the clonal diversity of γ9δ2T cell repertoires and characterized their target. We demonstrated that only a fraction of expanded γ9δ2T cells was active against cancer cells and that activity of the parental clone, or functional avidity of selected γ9δ2 T cell receptors ( ...[more]

PMID: 32484803

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