Dataset Information

Optogenetic Src Temporal Signaling

ABSTRACT: The purpose of this study was to characterize a novel optogenetic kinase system, a Light-Regulated (LightR) switch domain, designed to improve spatiotemporal control of signaling. Using LC-MS/MS, tyrosine phosphorylation was measured after 10 seconds, 30 seconds, 1 minute, 5 minutes and 60 minutes of LightR Src activation. TMT labeling was utilized to allow multiplexed relative quantification across time points. Results provide insight into the timing of Src and Src substrate activation.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jason Conage-Pough

LAB HEAD: Forest Michael White

PROVIDER: PXD018162 | Pride | 2020-10-20

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	011520_AC37_PC2_optosrc_repeat2_TMT11_supe.mzid	Mzid
	011520_AC37_PC2_optosrc_repeat3_TMT11_supe.mzid	Mzid
	011520_pY_spincolumn_AC10_optosrc_repeat3_TMT11plex.mzid	Mzid
	011520_pY_spincolumn_AC11_optosrc_repeat2_TMT11plex.mzid	Mzid
	030920_AC37_PC2_optosrc_zeroes_TMT6_supe.mzid	Mzid

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Publications

Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity.

Shaaya Mark M Fauser Jordan J Zhurikhina Anastasia A Conage-Pough Jason E JE Huyot Vincent V Brennan Martin M Flower Cameron T CT Matsche Jacob J Khan Shahzeb S Natarajan Viswanathan V Rehman Jalees J Kota Pradeep P White Forest M FM Tsygankov Denis D Karginov Andrei V AV

eLife 20200923

Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical limitations. Here, we report an optogenetic approach that utilizes an engineered Light-Regulated (LightR) allosteric switch module to achieve tight spatiotemporal control of enzymatic activity. Using the tyrosine kinase Src as a model, we demonstrat ...[more]

PMID: 32965214

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