Dataset Information

RAP-MS analysis of LncRNA BGL3 interacting proteins in cells

ABSTRACT: Long non-coding RNAs (lncRNAs) are emerging regulators of genomic stability and human disease. However, the molecular mechanisms by which nuclear lncRNAs directly contribute to DNA damage responses remain largely unknown. Using RNA antisense purification coupled with quantitative mass spectrometry (RAP-qMS), we found that the lncRNA BGL3 binds to PARP1 and BARD1, exhibiting unexpected roles in homologous recombination. Mechanistically, BGL3 is recruited to DNA double-strand breaks (DSBs) by PARP1 at an early time point, which requires its interaction with the DNA-binding domain of PARP1. BGL3 also binds the C-terminal BRCT domain and an internal region (amino acids 127-424) of BARD1, which mediates interaction of the BRCA1/BARD1 complex with its binding partners such as HP1γ and RAD51, resulting in BRCA1/BARD1 retention at DSBs. Cells depleted for BGL3 displayed genomic instability and were sensitive to DNA-damaging reagents.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Shaojie Mi

LAB HEAD: Huadong Pei

PROVIDER: PXD018186 | Pride | 2020-04-22

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	1_ProteinGroupsandPeptide.xlsx	Xlsx
	Fusion3_PHD_HZH-BGL3-1.raw	Raw
	Fusion3_PHD_HZH-BGL3-2.raw	Raw
	Fusion3_PHD_HZH-BGL3-3.raw	Raw
	Fusion3_PHD_HZH-RMRP-1.raw	Raw

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Publications

BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.

Hu Zhaohua Z Mi Shaojie S Zhao Ting T Peng Changmin C Peng Yihan Y Chen Lulu L Zhu Wenge W Yao Yi Y Song Qibin Q Li Xiangpan X Li Xinzhi X Jia Chenxi C Pei Huadong H

The EMBO journal 20200429 12

Long non-coding RNAs (lncRNAs) are emerging regulators of genomic stability and human disease. However, the molecular mechanisms by which nuclear lncRNAs directly contribute to DNA damage responses remain largely unknown. Using RNA antisense purification coupled with quantitative mass spectrometry (RAP-qMS), we found that the lncRNA BGL3 binds to PARP1 and BARD1, exhibiting unexpected roles in homologous recombination. Mechanistically, BGL3 is recruited to DNA double-strand breaks (DSBs) by PARP ...[more]

PMID: 32347575

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