Proteomics

Dataset Information

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Analysis of histone PTMs in nascent chromatin in new and old histones upon DNA damage (CAL353, CAL358) using the NCC-SILAC approach

ABSTRACT: Dynamic recycling and de novo deposition of histones are fundamental for chromatin restoration. Histone post-translational modifications (PTMs) are assumed to have a causal role in establishing distinct chromatin structures. However, it remains unknown how specific histone PTMs are regulated at broken replication fork. To get better insight into histone PTMs during DNA damage response, we combined NCC (Nascent chromatin capture) with SILAC-MS for identification of histone PTMs at replication forks upon CPT (camptothecin).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Cervix

SUBMITTER: Ignasi Forne  

LAB HEAD: Prof. Anja Groth

PROVIDER: PXD018220 | Pride | 2021-01-18

REPOSITORIES: Pride

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Publications

Proteome dynamics at broken replication forks reveal a distinct ATM-directed repair response suppressing DNA double-strand break ubiquitination.

Nakamura Kyosuke K   Kustatscher Georg G   Alabert Constance C   Hödl Martina M   Forne Ignasi I   Völker-Albert Moritz M   Satpathy Shankha S   Beyer Tracey E TE   Mailand Niels N   Choudhary Chunaram C   Imhof Axel A   Rappsilber Juri J   Groth Anja A  

Molecular cell 20210114 5

Cells have evolved an elaborate DNA repair network to ensure complete and accurate DNA replication. Defects in these repair machineries can fuel genome instability and drive carcinogenesis while creating vulnerabilities that may be exploited in therapy. Here, we use nascent chromatin capture (NCC) proteomics to characterize the repair of replication-associated DNA double-strand breaks (DSBs) triggered by topoisomerase 1 (TOP1) inhibitors. We reveal profound changes in the fork proteome, includin  ...[more]

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