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The Sperm Proteome of the Oyster Crassostrea hongkongensis

ABSTRACT: Here we reported 226 sperm proteins from the Hong Kong oyster Crassostrea hongkongensis. Proteins extracted from three sperm samples were separated by SDS-PAGE, analyzed by LC-MS/MS and identified using Mascot.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Crassostrea Hongkongensis

TISSUE(S): Spermatozoon, Sperm

SUBMITTER: Huawei MU

LAB HEAD: Qiu Jian-Wen

PROVIDER: PXD018255 | Pride | 2020-09-04

REPOSITORIES: Pride

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			Action	DRS
	SR1-1.msf	Msf
	SR1-1.raw	Raw
	SR1-2.msf	Msf
	SR1-2.raw	Raw
	SR1-3.msf	Msf

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The Sperm Proteome of the Oyster Crassostrea hongkongensis.

Mu Huawei H Ke Shengwei S Zhang Duo D Zhang Yanjie Y Song Xiaoyuan X Yu Ziniu Z Zhang Yang Y Qiu Jian-Wen JW

Proteomics 20201004 19-20

Sperm proteins play vital roles in fertilization, but little is known about their identities in free-spawning marine invertebrates. Here, 286 sperm proteins are reported from the Hong Kong oyster Crassostrea hongkongensis using label-free and semi-quantitative proteomics. Proteins extracted from three sperm samples are separated by SDS-PAGE, analyzed by LC-MS/MS, and identified using Mascot. Functional classification of the sperm proteome reveals energy metabolism (33%), signaling and binding (2 ...[more]

PMID: 32865869

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