Proteomics

Dataset Information

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Quantitative proteomic analysis of ARPE-19 cells in response to LPS

ABSTRACT: Overwhelming and persistent inflammation of retinal pigment epithelium (RPE) induces destructive changes in retinal environment by invoking immune activation. In this study, we aimed to investigate RPE-specific biological and metabolic response against intense inflammation, and identify molecular characteristics determining pathological progression. Here, we performed quantitative analyses of proteome and phosphoproteome of lipopolysaccharide (LPS)-stimulated human derived RPE cell line ARPE-19 using the latest isobaric TMT labeling approach coupled with high-resolution mass spectrometry.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Retinal Pigment Epithelium Cell Line, Epithelial Cell

SUBMITTER: Dohyun Han  

LAB HEAD: Dohyun Han

PROVIDER: PXD018257 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20191212_ARPE19_LPS_TMT10_F.msf Msf
20191212_ARPE19_LPS_TMT10_F.pdResult Other
20191212_ARPE19_LPS_TMT10_F1.raw Raw
20191212_ARPE19_LPS_TMT10_F10.raw Raw
20191212_ARPE19_LPS_TMT10_F11.raw Raw
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Publications

A Comprehensive Proteomic and Phosphoproteomic Analysis of Retinal Pigment Epithelium Reveals Multiple Pathway Alterations in Response to the Inflammatory Stimuli.

Song Juha J   Han Dohyun D   Lee Heonyi H   Kim Da Jung DJ   Cho Joo-Youn JY   Park Jae-Hak JH   Seok Seung Hyeok SH  

International journal of molecular sciences 20200425 9

Overwhelming and persistent inflammation of retinal pigment epithelium (RPE) induces destructive changes in the retinal environment. However, the precise mechanisms remain unclear. In this study, we aimed to investigate RPE-specific biological and metabolic responses against intense inflammation and identify the molecular characteristics determining pathological progression. We performed quantitative analyses of the proteome and phosphoproteome of the human-derived RPE cell line ARPE-19 after tr  ...[more]

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