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Proteome-scale correlation analysis between urinary extracellular vesicle (uEV) and kidney

ABSTRACT: We profiled the proteomes of uEV and kidney from twelve rats. Six of them were under a standard diet while the other six under a high potassium diet for 4 days. During the revision stage of this project, we further put five rats in metabolic cages for two days, fed with the high potassium diet for 2 days. We made proteome-scale correlation analyses of uEV and kidney using absolute label-free quantification algorithm iBAQ under these three conditions: control, high K 4day, and high K 2 day. We also did label-free quantification (LFQ) to investigate the protein abundance changes in uEV and kidney between control and high K 4day.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Urine, Whole Body

SUBMITTER: Qi Wu

LAB HEAD: Robert A. Fenton

PROVIDER: PXD018363 | Pride | 2021-07-27

REPOSITORIES: Pride

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			Action	DRS
	EXOSOMES-1-1.raw	Raw
	EXOSOMES-1-1_171130035716.raw	Raw
	EXOSOMES-1-2.raw	Raw
	EXOSOMES-1-2_171130052653.raw	Raw
	EXOSOMES-1-3.raw	Raw

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Publications

Large-Scale Proteomic Assessment of Urinary Extracellular Vesicles Highlights Their Reliability in Reflecting Protein Changes in the Kidney.

Wu Qi Q Poulsen Søren B SB Murali Sathish K SK Grimm Paul R PR Su Xiao-Tong XT Delpire Eric E Welling Paul A PA Ellison David H DH Ellison David H DH Fenton Robert A RA

Journal of the American Society of Nephrology : JASN 20210706 9

<h4>Background</h4>Urinary extracellular vesicles (uEVs) are secreted into urine by cells from the kidneys and urinary tract. Although changes in uEV proteins are used for quantitative assessment of protein levels in the kidney or biomarker discovery, whether they faithfully reflect (patho)physiologic changes in the kidney is a matter of debate.<h4>Methods</h4>Mass spectrometry was used to compare in an unbiased manner the correlations between protein levels in uEVs and kidney tissue from the sa ...[more]

PMID: 34230103

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