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Mycobacteria tolerate carbon monoxide by remodelling their respiratory chain

ABSTRACT: Carbon monoxide (CO) is a gas infamous for its acute toxicity. The toxicity of CO predominately stems from its tendency to form carbonyl complexes with transition metals, thus inhibiting the heme-prosthetic groups of proteins, including the terminal oxidases of the respiratory chain. While CO has been proposed as an antibacterial agent, the evidence supporting its toxicity towards bacteria is equivocal, and its cellular targets remain poorly defined. In this work, we investigate the physiological response of Mycobacterium smegmatis to CO. We show that M. smegmatis is highly resistant to the toxic effects of CO, exhibiting normal growth parameters when cultured in its presence. We profiled the proteome of M. smegmatis during growth in CO, identifying strong induction of cytochrome bd oxidase and members of the dos regulon, but relatively few other changes. We show that the activity of cytochrome bd oxidase is resistant to CO, whereas cytochrome bcc-aa3 oxidase is strongly inhibited by this gas. Consistent with these findings, growth analysis shows that M. smegmatis lacking cytochrome bd oxidase displays a significant growth defect in the presence of CO, while induction of the dos regulon appears to be unimportant for adaption to CO. Altogether, our findings suggest that M. smegmatis has considerable resistance to CO and benefits from respiratory flexibility to withstand its inhibitory effects.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Mycobacterium Sp. Gn-10803

SUBMITTER: Cheng Huang

LAB HEAD: Chris Greening

PROVIDER: PXD018382 | Pride | 2021-04-30

REPOSITORIES: Pride

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	F1CH20190327_C_1.raw	Raw
	F1CH20190327_C_2.raw	Raw
	F1CH20190327_C_3.raw	Raw
	F1CH20190327_C_4.raw	Raw
	F1CH20190327_C_5.raw	Raw

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