Dataset Information

TECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples

ABSTRACT: High-throughput has become a substantial need in many omics investigations. In proteomics the sample preparation workflow consists of multiple steps, whereby with each additional manual step more bias is added to the sample. Especially for label-free quantification experiments this drastically reduces the outcome. Here, a positive pressure workstation was evaluated to increase automation of sample preparation and decrease input time and consumables. The semi-automated workflow with the Resolvex A200 workstation including the sorbents Maestro and WWP2 performed equally well to the routinely conducted manual workflow, with similar technical variability in MSMS-based identifications of peptides and proteins. Both sorbents produced highly reproducible results within a range of 10-300 µg of peptide starting material. A direct, on-column sample preparation using the NBE columns - without conditioning and equilibration of the sorbents -works generally robust, whereby consumables and time are drastically reduced.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Suspension Culture, Permanent Cell Line Cell

SUBMITTER: Louise Schelletter

LAB HEAD: Thomas Noll

PROVIDER: PXD018444 | Pride | 2021-01-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ExperimentalDesignTemplate_A_withDescription.txt	Txt
	ExperimentalDesignTemplate_B_withDescription.txt	Txt
	ExperimentalDesignTemplate_C_withDescription.txt	Txt
	FASTA.rar	Other
	Raw_A.rar	Other

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Publications

A positive pressure workstation for semi-automated peptide purification of complex proteomic samples.

Schelletter Louise L Hertel Oliver O Antar Shareef Jarvi SJ Scherling Christian C Lättig Jens J Noll Thomas T Hoffrogge Raimund R

Rapid communications in mass spectrometry : RCM 20200817 2

<h4>Rationale</h4>High-throughput reliable data generation has become a substantial requirement in many "omics" investigations. In proteomics the sample preparation workflow consists of multiple steps adding more bias to the sample with each additional manual step. Especially for label-free quantification experiments, this drastically impedes reproducible quantification of proteins in replicates. Here, a positive pressure workstation was evaluated to increase automation of sample preparation and ...[more]

PMID: 32583429

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