Dataset Information

Label free proteomics analysis of differentiating human muscle cells.

ABSTRACT: We have carried out systematic analysis of protein expression changes during differentiation of human myoblasts into myotubes over 6 days period in cell culture.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Activated Skeletal Muscle Satellite Cell

SUBMITTER: Adelina Rogowska-Wrzesinska

LAB HEAD: Adelina Rogowska-Wrzesinska

PROVIDER: PXD018588 | Pride | 2020-05-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170426_HumanProteome_Reviewed_20198Proteins_FromWojciech.fasta	Fasta
	LUM_02362_KS__X111_M51_col3_200nlMin_1.raw	Raw
	LUM_02363_KS__X111_M51_col3_200nlMin_2.raw	Raw
	LUM_02364_KS__X111_M51_col3_200nlMin_3.raw	Raw
	LUM_02365_KS__X114_M54_col3_200nlMin_1.raw	Raw

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Publications

PolySTest: Robust Statistical Testing of Proteomics Data with Missing Values Improves Detection of Biologically Relevant Features.

Schwämmle Veit V Hagensen Christina E CE Rogowska-Wrzesinska Adelina A Jensen Ole N ON

Molecular & cellular proteomics : MCP 20200518 8

Statistical testing remains one of the main challenges for high-confidence detection of differentially regulated proteins or peptides in large-scale quantitative proteomics experiments by mass spectrometry. Statistical tests need to be sufficiently robust to deal with experiment intrinsic data structures and variations and often also reduced feature coverage across different biological samples due to ubiquitous missing values. A robust statistical test provides accurate confidence scores of larg ...[more]

PMID: 32424025

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