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Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer’s Disease Progression

ABSTRACT: The molecular networks underlying Alzheimer’s disease (AD) are not well-defined. We present temporal profiling of >14,000 proteins and >34,000 phosphosites at the asymptomatic and symptomatic stages of AD, deep proteomics analysis of transgenic mouse models.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Yuxin Li

LAB HEAD: Junmin Peng

PROVIDER: PXD018590 | Pride | 2020-04-16

REPOSITORIES: Pride

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			Action	DRS
	ad_b1_whp_f10_01.1.pepXML	Pepxml
	ad_b1_whp_f10_01.raw	Raw
	ad_b1_whp_f11_01.1.pepXML	Pepxml
	ad_b1_whp_f11_01.raw	Raw
	ad_b1_whp_f12_02.1.pepXML	Pepxml

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Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression.

Bai Bing B Wang Xusheng X Li Yuxin Y Chen Ping-Chung PC Yu Kaiwen K Dey Kaushik Kumar KK Yarbro Jay M JM Han Xian X Lutz Brianna M BM Rao Shuquan S Jiao Yun Y Sifford Jeffrey M JM Han Jonghee J Wang Minghui M Tan Haiyan H Shaw Timothy I TI Cho Ji-Hoon JH Zhou Suiping S Wang Hong H Niu Mingming M Mancieri Ariana A Messler Kaitlynn A KA Sun Xiaojun X Wu Zhiping Z Pagala Vishwajeeth V High Anthony A AA Bi Wenjian W Zhang Hui H Chi Hongbo H Haroutunian Vahram V Zhang Bin B Beach Thomas G TG Yu Gang G Peng Junmin J

Neuron 20200108 6

Alzheimer's disease (AD) displays a long asymptomatic stage before dementia. We characterize AD stage-associated molecular networks by profiling 14,513 proteins and 34,173 phosphosites in the human brain with mass spectrometry, highlighting 173 protein changes in 17 pathways. The altered proteins are validated in two independent cohorts, showing partial RNA dependency. Comparisons of brain tissue and cerebrospinal fluid proteomes reveal biomarker candidates. Combining with 5xFAD mouse analysis, ...[more]

PMID: 31926610

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