Proteomics

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Sphingobium indicum B90A nLC-MS/MS

ABSTRACT: The project aims to to understand the response of the lin genes in Sphingobium indicum B90A under the stress of HCH isomers and the metabolites formed during degradation of hexachlorocyclohexane (HCH). Entire cell proteome from Sphingobium indicum B90A was extracted in presence of four HCH isomers. Quantitative proteomics confirmed the constitutive expression of the linA, linB and linC genes of the HCH degradation pathway crucial for the initiation of HCH isomers degradation. LinM and LinN were upregulated in the presence of β- and δ-isomers suggested the important role of ABC transporter system in the depletion of β- and δ-HCH. Besides this HCH isomers induced oxidative stress caused systemic changes in strain B90A proteome.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Sphingobium Indicum B90a

TISSUE(S): Cell Culture

SUBMITTER: RUP LAL  

LAB HEAD: Rup Lal

PROVIDER: PXD018599 | Pride | 2021-04-06

REPOSITORIES: Pride

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Publications

Comparative proteomics unravelled the hexachlorocyclohexane (HCH) isomers specific responses in an archetypical HCH degrading bacterium Sphingobium indicum B90A.

Sharma Monika M   Singh Durgesh Narain DN   Budhraja Rohit R   Sood Utkarsh U   Rawat Charu Dogra CD   Adrian Lorenz L   Richnow Hans-Hermann HH   Singh Yogendra Y   Negi Ram Krishan RK   Lal Rup R  

Environmental science and pollution research international 20210330 30

Hexachlorocyclohexane (HCH) is a persistent organochlorine pesticide that poses threat to different life forms. Sphingobium indicum B90A that belong to sphingomonad is well-known for its ability to degrade HCH isomers (α-, β-, γ-, δ-), but effects of HCH isomers and adaptive mechanisms of strain B90A under HCH load remain obscure. To investigate the responses of strain B90A to HCH isomers, we followed the proteomics approach as this technique is considered as the powerful tool to study the micro  ...[more]

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