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MS analysis of SARS-CoV2 proteins from patient samples

ABSTRACT: We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. https://biorxiv.org/cgi/content/short/2020.04.18.047878v1

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Severe Acute Respiratory Syndrome

SUBMITTER: Christian Ihling

LAB HEAD: Andrea Sinz

PROVIDER: PXD018682 | Pride | 2020-04-24

REPOSITORIES: Pride

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			Action	DRS
	02.mzML	Mzml
	02.pride.mgf.gz	Mgf
	02.raw	Raw
	03.mzML	Mzml
	03.pride.mgf.gz	Mgf

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MS analysis of SARS-CoV2 proteins from patient samples

Project description:We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. https://biorxiv.org/cgi/content/short/2020.04.18.047878v1

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