Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration
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ABSTRACT: This study sought to explore phosphoproteome signaling dysregulation in different models of neurodegeneration. We collected phosphotyrosine, MAPK/CDK substrates, global phosphoproteome, and protein expression data from hippocampus and/or cortex tissue from each model. Tryptic peptides were multiplexd with TMT, enriched for phosphopeptides, and then identified and quantified by LC-MS/MS. We additionally quantified phosphotyrosine peptides from BV-2 cells expressing various Siglec receptor constructs.
INSTRUMENT(S): LTQ Orbitrap, Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain, Cell Culture
SUBMITTER: Nader Morshed
LAB HEAD: Forest Michael White
PROVIDER: PXD018757 | Pride | 2020-11-09
REPOSITORIES: Pride
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