Proteomics

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Label-Free Proteomics Profiling of Nanovesicles Isolated from Cultured Salivary Glands Isolated from Partially-Fed Adult Female Ixodes scapularis


ABSTRACT: Ticks are ectoparasites that have co-evolved with their hosts for millions of years. Unlike other blood-feeders, hard ticks take several days to complete a blood-meal, which makes them especially susceptible to the detection by host immune responses. To overcome this, ticks have developed a vast array of salivary effectors with immune modulatory properties. These effectors not only benefit tick survival but also influence pathogen transmission. We have characterized the extracellular vesicle protein cargo secreted within tick saliva. Extracellular vesicles were purified through density gradient from salivary gland cultures dissected from partially fed adult female Ixodes scapularis. Molecules present in the vesicles were then characterized using label-free proteomics. In this protein dataset, we have identified several tick immune modulatory proteins. This dataset demonstrates that arthropods secrete extracellular vesicles for the translocation of effectors during blood-feeding.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Ixodes Scapularis (black-legged Tick) (deer Tick)

TISSUE(S): Saliva-secreting Gland, Salivary Gland

DISEASE(S): Wounds And Injuries

SUBMITTER: Cristina Clement  

LAB HEAD: Joao Pedra

PROVIDER: PXD018779 | Pride | 2021-06-07

REPOSITORIES: Pride

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