Dataset Information

Proteome responses of Grapevine to Downy mildew

ABSTRACT: A comparative proteomic analysis of grapevine leaves from the resistant genotype V. davidii ‘LiuBa-8’ (LB) and susceptible genotype V. vinifera ‘Pinot Noir’ (PN) at 12 hpi was conducted to understand the complex relationship between grapevine and P. viticola and the molecular mechanisms between difference resistant vitis genotypes at the early stage of infection. A total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN respectively at 12 hpi by iTRAQ. MapMan analysis showed that the majority of these DEPs were related to photosynthesis, metabolism, stress and redox. More up-expressed DEPs involved in photosynthesis and less down-expressed DEPs involved in metabolism contribute to the resistance in LB. PR10.2, PR10.3, HSF70.2 and HSP90.6 are proposed to be key proteins in both compatible and incompatible interactions. Accumulation H2O2 established the ROS signaling in incompatible interaction and APXs, GSTs maybe associated with the resistance of grapevine against downy mildew. Moreover, we verified four proteins to ensure the accuracy of proteome data using PRM. Overall, these data provide new insights into molecular events and provide valuable candidate proteins that could be used to illuminate molecular mechanisms underlying the incompatible and compatible interaction in early stage and eventually to be exploited to develop new protection strategy against downy mildew in grapevine.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Leaf

SUBMITTER: Guotian Liu

LAB HEAD: Yan Xu

PROVIDER: PXD018868 | Pride | 2021-09-09

REPOSITORIES: Pride

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Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.

Liu Guo-Tian GT Wang Bian-Bian BB Lecourieux David D Li Mei-Jie MJ Liu Ming-Bo MB Liu Rui-Qi RQ Shang Bo-Xing BX Yin Xiao X Wang Li-Jun LJ Lecourieux Fatma F Xu Yan Y

Horticulture research 20210501 1

Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii "LiuBa-8" (LB) and susceptible V. vinifera "Pinot Noir" (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identifi ...[more]

PMID: 33931609

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